College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
J Exp Bot. 2011 Nov;62(15):5659-69. doi: 10.1093/jxb/err252. Epub 2011 Aug 26.
In order to characterize the potential transcriptional regulation of core components of abscisic acid (ABA) signal transduction in tomato fruit development and drought stress, eight SlPYL (ABA receptor), seven SlPP2C (type 2C protein phosphatase), and eight SlSnRK2 (subfamily 2 of SNF1-related kinases) full-length cDNA sequences were isolated from the tomato nucleotide database of NCBI GenBank. All SlPYL, SlPP2C, and SlSnRK2 genes obtained are homologous to Arabidopsis AtPYL, AtPP2C, and AtSnRK2 genes, respectively. Based on phylogenetic analysis, SlPYLs and SlSnRK2s were clustered into three subfamilies/subclasses, and all SlPP2Cs belonged to PP2C group A. Within the SlPYL gene family, SlPYL1, SlPYL2, SlPYL3, and SlPYL6 were the major genes involved in the regulation of fruit development. Among them, SlPYL1 and SlPYL2 were expressed at high levels throughout the process of fruit development and ripening; SlPYL3 was strongly expressed at the immature green (IM) and mature green (MG) stages, while SlPYL6 was expressed strongly at the IM and red ripe (RR) stages. Within the SlPP2C gene family, the expression of SlPP2C, SlPP2C3, and SlPP2C4 increased after the MG stage; SlPP2C1 and SlPP2C5 peaked at the B3 stage, while SlPP2C2 and SlPP2C6 changed little during fruit development. Within the SlSnRK2 gene family, the expression of SlSnRK2.2, SlSnRK2.3, SlSnRK2.4, and SlSnRK2C was higher than that of other members during fruit development. Additionally, most SlPYL genes were down-regulated, while most SlPP2C and SlSnRK2 genes were up-regulated by dehydration in tomato leaf.
为了研究番茄果实发育和干旱胁迫过程中脱落酸（ABA）信号转导核心成分的潜在转录调控，我们从 NCBI GenBank 的番茄核苷酸数据库中分离出了 8 个 SlPYL（ABA 受体）、7 个 SlPP2C（2C 型蛋白磷酸酶）和 8 个 SlSnRK2（SNF1 相关激酶亚家族 2）全长 cDNA 序列。所有获得的 SlPYL、SlPP2C 和 SlSnRK2 基因均与拟南芥 AtPYL、AtPP2C 和 AtSnRK2 基因同源。基于系统发育分析，SlPYLs 和 SlSnRK2s 被聚类为 3 个亚家族/类，所有 SlPP2Cs 均属于 PP2C 组 A。在 SlPYL 基因家族中，SlPYL1、SlPYL2、SlPYL3 和 SlPYL6 是调节果实发育的主要基因。其中，SlPYL1 和 SlPYL2 在果实发育和成熟过程中表达水平较高；SlPYL3 在未成熟绿（IM）和成熟绿（MG）期表达强烈，而 SlPYL6 在 IM 和红熟（RR）期表达强烈。在 SlPP2C 基因家族中，SlPP2C、SlPP2C3 和 SlPP2C4 的表达在 MG 期后增加；SlPP2C1 和 SlPP2C5 在 B3 期达到峰值，而 SlPP2C2 和 SlPP2C6 在果实发育过程中变化不大。在 SlSnRK2 基因家族中，SlSnRK2.2、SlSnRK2.3、SlSnRK2.4 和 SlSnRK2C 的表达在果实发育过程中高于其他成员。此外，番茄叶片脱水时，大多数 SlPYL 基因下调，而大多数 SlPP2C 和 SlSnRK2 基因上调。
