Sakbun V, Ali S M, Lee Y A, Jara C S, Bryant-Greenwood G D
Department of Anatomy and Reproductive Biology, University of Hawaii, Honolulu 96822.
Am J Obstet Gynecol. 1990 May;162(5):1310-7. doi: 10.1016/0002-9378(90)90043-7.
Human placental lactogen is one of the major hormones secreted by the placental syncytiotrophoblast and detected in the maternal circulation. Other sources of this hormone in intrauterine tissues at term have been sought by means of immunohistochemistry and northern analysis. Avidin-biotin immunoperoxidase staining with a specific polyclonal antibody to human placental lactogen showed this hormone to be present in groups of cells at the interface between chorionic cytotrophoblast and decidua parietalis and in some cells of the basal plate in addition to the classic source, the syncytiotrophoblast. Hybridization of polyadenylic-(+)ribonucleic acid extracted from amnion, chorion, decidua parietalis, basal plate, and placental trophoblast with a radiolabeled 48 mer oligonucleotide and a 540 base pair complementary deoxyribonucleic acid probe to human placental lactogen showed the placental trophoblast to be the major source of human placental lactogen and the extravillous chorion and basal plate to be additional minor sources.
人胎盘催乳素是胎盘合体滋养层分泌并在母体循环中检测到的主要激素之一。已通过免疫组织化学和Northern分析寻找足月时子宫内组织中这种激素的其他来源。用针对人胎盘催乳素的特异性多克隆抗体进行抗生物素蛋白-生物素免疫过氧化物酶染色显示，除了经典来源合体滋养层外，这种激素还存在于绒毛膜细胞滋养层与子宫壁蜕膜之间界面处的细胞群以及基底板的一些细胞中。从羊膜、绒毛膜、子宫壁蜕膜、基底板和胎盘滋养层中提取的聚腺苷酸-（+）核糖核酸与放射性标记的48聚体寡核苷酸和与人胎盘催乳素互补的540碱基对脱氧核糖核酸探针杂交，结果显示胎盘滋养层是人胎盘催乳素的主要来源，而绒毛外绒毛膜和基底板是另外的次要来源。
