Schindzielorz A H, Belshe R B, Mufson M A
Marshall University School of Medicine, Huntington, West Virginia.
Am J Trop Med Hyg. 1990 May;42(5):460-4. doi: 10.4269/ajtmh.1990.42.460.
To further characterize HIV-1 and HIV-2 Western blot indeterminate (IWB) sera, 402 sera from 318 healthy low-risk individuals from West Virginia and 159 African sera obtained in the pre-AIDS era (1968-1972) were studied. All IWB sera tested for antigen by HIV-1 enzyme immunoassay (EIA-Ag) were negative. HIV-1 and HIV-2 IWB reactivity occurred independent of HIV-1 and HIV-2 false-positive testing for antibody by enzyme immunoassay (EIA-Ab) and no cross-reactions between HIV-1 and HIV-2 IWB patterns were detected. The IWB patterns were reproducible, demonstrated no age or sex related pattern, and showed no evidence of vertical or horizontal transmission. The African sera exhibited a significantly higher number of IWB patterns. IWB reactivity in HIV-1 and HIV-2 seronegative individuals may not be viral in origin and the occurrence of IWB pattern may vary among populations.
为进一步鉴定HIV-1和HIV-2免疫印迹不确定（IWB）血清，对来自西弗吉尼亚州318名健康低风险个体的402份血清以及在艾滋病流行前时代（1968 - 1972年）获得的159份非洲血清进行了研究。通过HIV-1酶免疫测定法（EIA-Ag）检测抗原的所有IWB血清均为阴性。HIV-1和HIV-2 IWB反应性的出现与通过酶免疫测定法（EIA-Ab）检测抗体时HIV-1和HIV-2的假阳性检测无关，并且未检测到HIV-1和HIV-2 IWB模式之间的交叉反应。IWB模式具有可重复性，未显示与年龄或性别相关的模式，也没有垂直或水平传播的证据。非洲血清呈现出明显更多数量的IWB模式。HIV-1和HIV-2血清阴性个体中的IWB反应性可能并非源于病毒，并且IWB模式的出现可能因人群而异。
