Research Institute of Atherosclerotic Disease, Xi'an Jiaotong University School of Medicine, Xi'an, China.
J Thromb Thrombolysis. 2012 May;33(4):301-7. doi: 10.1007/s11239-011-0627-0.
Several lines of evidence have shown that plasma C-reactive protein (CRP) is associated with increased risk of stroke; however, previous studies were not adequately powered to assess whether plasma CRP levels are associated with stroke progression. In the current study, we designed a rabbit stroke model and investigated the relationship between plasma CRP and infarcted brain tissue. To produce a rabbit stroke model, we injected autologous thrombi into the left internal carotid artery. The plasma CRP levels were measured by ELISA at 0.5, 3, 6, 9, and 12 h poststroke. At 12 h, the rabbits were sacrificed, and the whole brains were examined by H & E and immunohistochemical staining with a monoclonal antibody against rabbit CRP. CRP mRNA expression in the infarcted tissue was evaluated by RT-PCR. Plasma CRP was markedly increased after embolic stroke. Plasma CRP positively correlated with the cerebral infarct area (r = 0.98, P < 0.01). Immunohistochemical staining revealed that CRP was frequently present in the infarcted area but not in normal cerebral tissue. RT-PCR showed that CRP was expressed in infarcted brain tissue. The plasma CRP level was significantly elevated after stroke and was closely correlated with the size of infarction, suggesting that CRP is an ideal marker to assess the acute embolic stroke.
已有数条证据表明，血浆 C 反应蛋白（CRP）与中风风险增加有关；然而，之前的研究没有足够的能力来评估血浆 CRP 水平是否与中风进展有关。在本研究中，我们设计了一个兔中风模型，并研究了血浆 CRP 与梗死脑组织之间的关系。为了产生兔中风模型，我们将自体血栓注入左侧颈内动脉。通过 ELISA 在中风后 0.5、3、6、9 和 12 小时测量血浆 CRP 水平。在 12 小时时，处死兔子，用 H&E 和针对兔 CRP 的单克隆抗体进行免疫组织化学染色检查整个大脑。通过 RT-PCR 评估梗死组织中的 CRP mRNA 表达。栓塞性中风后，血浆 CRP 明显增加。血浆 CRP 与脑梗死面积呈正相关（r=0.98，P<0.01）。免疫组织化学染色显示 CRP 经常存在于梗死区而不存在于正常脑组织中。RT-PCR 显示 CRP 在梗死脑组织中表达。中风后血浆 CRP 水平显著升高，与梗死面积密切相关，表明 CRP 是评估急性栓塞性中风的理想标志物。
