哺乳动物皮肤的表皮通透性屏障测量
Epidermal permeability barrier measurement in mammalian skin.
作者信息
Indra Arup Kumar, Leid Mark
机构信息
Department of Pharmaceutical Sciences, College of Pharmacy, and Environmental Health Sciences Center, Oregon State University, Corvallis, OR, USA.
出版信息
Methods Mol Biol. 2011;763:73-81. doi: 10.1007/978-1-61779-191-8_4.
Abstract
A defective skin epidermal permeability barrier (EPB) is responsible for a high mortality rate in premature infants and is an important risk factor in inflammatory skin diseases such as eczema. We report here fast and accurate methods for measurement of EPB in animal models or in human patients using simple techniques that monitor diffusion of dyes (X-Gal or Lucifer Yellow) through the upper epidermis and measure transepidermal water loss (TEWL) resulting from a defective skin barrier. Accurate diagnosis and early detection of EPB defects in human patients are critical for effective treatment of certain classes of inflammatory skin diseases.
摘要
有缺陷的皮肤表皮渗透屏障（EPB）是导致早产儿高死亡率的原因，也是湿疹等炎症性皮肤病的重要风险因素。我们在此报告了在动物模型或人类患者中测量EPB的快速准确方法，这些方法使用简单技术监测染料（X-Gal或荧光素黄）透过表皮上层的扩散，并测量因皮肤屏障缺陷导致的经表皮水分流失（TEWL）。准确诊断和早期发现人类患者的EPB缺陷对于有效治疗某些类型的炎症性皮肤病至关重要。
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本文引用的文献
1
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Skin Res Technol. 2011 Aug;17(3):366-72. doi: 10.1111/j.1600-0846.2011.00509.x. Epub 2011 Apr 15.
2
Making an epidermis.制造表皮。
Ann N Y Acad Sci. 2009 Jul;1170:7-10. doi: 10.1111/j.1749-6632.2009.04363.x.
3
Dual role of COUP-TF-interacting protein 2 in epidermal homeostasis and permeability barrier formation.COUP-TF相互作用蛋白2在表皮稳态和通透性屏障形成中的双重作用。
J Invest Dermatol. 2009 Jun;129(6):1459-70. doi: 10.1038/jid.2008.392. Epub 2008 Dec 18.
4
"Outside-to-inside" (and now back to "outside") pathogenic mechanisms in atopic dermatitis.特应性皮炎中“由外向内”（现在又回到“向外”）的致病机制。
J Invest Dermatol. 2008 May;128(5):1067-70. doi: 10.1038/jid.2008.88.
5
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J Allergy Clin Immunol. 2008 Jun;121(6):1337-43. doi: 10.1016/j.jaci.2008.01.022. Epub 2008 Mar 7.
6
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J Lipid Res. 2008 Apr;49(4):697-714. doi: 10.1194/jlr.R800002-JLR200. Epub 2008 Feb 2.
7
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9
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Development. 2005 Oct;132(20):4533-44. doi: 10.1242/dev.02019.
10
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