Department of Environmental Sciences and Biotechnology, Hallym University, 1 Okchon, Chuncheon, Gangwon, Korea.
Bioprocess Biosyst Eng. 2012 Mar;35(3):441-8. doi: 10.1007/s00449-011-0583-2. Epub 2011 Aug 28.
In a complete nitrification sequencing batch reactor (CNSBR), where ammonium containing wastewater (200-1,000 mg N/L) is completely oxidized to nitrate up to 2.4 kg NH(4) (+)-N/m(3) d, both ammonia oxidizers and nitrite oxidizers were enriched in the sludge granules. Quantitative fluorescence in situ hybridization analyses of the sludge granules of the CNSBR showed that ammonia oxidizers and nitrite oxidizers occupied 31 and 4.2% of total bacteria, respectively. Most of the nitrite oxidizers were Nitrobacter species (95% of the nitrite oxidizers) and the remainder was Nitrospira species. The population of nitrite oxidizers was significantly higher than that of partial nitrification SBR (PNSBR) where most of the ammonium was oxidized to nitrite. The PNSBR had 37% (ammonia oxidizers) and 0.4% (nitrite oxidizers) of total bacteria. Comparative study with CNSBR and PNSBR revealed that free nitrous acid, rather than free ammonia, played a critical inhibition role to wash out nitrite oxidizers from the reactor. The concentrations of free ammonia and nitrite as well as free nitrous acid in the CNSBR selected Nitrobacter as the dominant nitrite oxidizers rather than Nitrospira.
在完全硝化序批式反应器（CNSBR）中，含氨废水（200-1000mgN/L）被完全氧化为硝酸盐，氨氮去除速率高达 2.4kgNH(4)(+)-N/m(3)d，氨氧化菌和亚硝酸盐氧化菌在污泥颗粒中得到富集。对 CNSBR 污泥颗粒的定量荧光原位杂交分析表明，氨氧化菌和亚硝酸盐氧化菌分别占总细菌的 31%和 4.2%。大多数亚硝酸盐氧化菌为硝化杆菌属（亚硝酸盐氧化菌的 95%），其余为硝化螺菌属。亚硝酸盐氧化菌的数量明显高于部分硝化 SBR（PNSBR），PNSBR 中大部分氨被氧化为亚硝酸盐。PNSBR 中氨氧化菌占总细菌的 37%，亚硝酸盐氧化菌占 0.4%。与 CNSBR 和 PNSBR 的比较研究表明，游离亚硝酸而不是游离氨对亚硝酸盐氧化菌具有关键的抑制作用，从而将其从反应器中冲洗出来。CNSBR 中的游离氨、亚硝酸盐和游离亚硝酸浓度选择硝化杆菌作为优势亚硝酸盐氧化菌，而不是硝化螺菌。
