Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu, Fukuoka, Japan.
Waste Manag. 2010 Jan;30(1):100-9. doi: 10.1016/j.wasman.2009.08.018. Epub 2009 Oct 6.
The nitrification of ammonium-rich wastewater is considered challenging due to the substrate inhibition particularly in the form of free ammonia (FA) and free nitrous acid (FNA) in ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB). The feasibility of the nitrifying activated sludge system to completely nitrify synthetic stabilized landfill leachate with N-NH(4)(+) concentration of 1452mg/L was tested in this study. The process started with 0.4kg N-NH(4)(+)/m(3)/day of nitrogen loading rate (NLR) in a fed-batch mode to avoid any accumulation of the FA and FNA in the system followed by increasing the nitrogen loading rate (NLR) gradually. Complete nitrification was achieved with a very high ammonium removal percentage (approximately 100%). The maximum specific and volumetric nitrification rate obtained were 0.49g N-NH(4)(+)/g VSS/day and 3.0kg N-NH(4)(+)/m(3)/day, respectively which were higher than those reported previously for ammonium-rich removal using activated sludge system. The nitrifying sludge exhibited good settling characteristics of up to 36mL/g VSS and a long SRT of more than 53 days which contributed to the success of the nitrification process. The coexistence and syntrophic association of the AOB and NOB was observed by using Fluorescence in situ hybridization (FISH) technique which supported the results on complete nitrification obtained in the system. These findings would be of prominent importance for further treatment of actual sanitary landfill leachate.
富含铵的废水的硝化作用被认为具有挑战性，特别是在氨氧化菌（AOB）和亚硝酸盐氧化菌（NOB）中以游离氨（FA）和游离亚硝酸（FNA）的形式存在时会对其产生基质抑制。本研究测试了硝化活性污泥系统将 1452mg/L 的 N-NH(4)(+)浓度的合成稳定垃圾渗滤液完全硝化的可行性。该过程从 0.4kg N-NH(4)(+)/m(3)/day 的氮负荷（NLR）开始分批进料模式，以避免系统中 FA 和 FNA 的任何积累，然后逐渐增加氮负荷（NLR）。完全硝化，铵去除率高达 100%左右。获得的最大比和容积硝化速率分别为 0.49g N-NH(4)(+)/g VSS/day 和 3.0kg N-NH(4)(+)/m(3)/day，高于先前使用活性污泥系统处理富含铵废水的报道。硝化污泥表现出良好的沉降特性，可达 36mL/g VSS，SRT 超过 53 天，这有助于硝化过程的成功。通过荧光原位杂交（FISH）技术观察到 AOB 和 NOB 的共存和共营养关联，这支持了系统中获得的完全硝化的结果。这些发现对于实际卫生填埋场渗滤液的进一步处理具有重要意义。
