C（6）-神经酰胺通过产生内源性神经酰胺增强枯否细胞的吞噬活性。
C(6)-ceramide enhances phagocytic activity of Kupffer cells through the production of endogenous ceramides.
机构信息
Department of Environmental and Health Chemistry, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
出版信息
Mol Cells. 2011 Oct;32(4):325-31. doi: 10.1007/s10059-011-1034-2. Epub 2011 Aug 25.
Ceramide has been suggested to be not only a tumor-suppressive lipid but also a regulator of phagocytosis. We examined whether exogenous cell-permeable C(6)-ceramide enhances the phagocytic activity of Kupffer cells (KCs) and affects the level of cellular ceramides. Rat KCs were isolated by collagenase digestion and differential centrifugation, using Percoll system. Phagocytic activity was measured by FACS analysis after incubating KCs with fluorescence-conjugated latex beads, and the level of cellular ceramide was analyzed by liquid chromatography tandem-mass spectrometry (LC-MS/MS). In this study we found that permeable C(6)-ceramide increases the cellular levels of endogenous ceramides via a sphingosine-recycling pathway leading to enhanced phagocytosis by KCs.
神经酰胺不仅被认为是一种肿瘤抑制脂质，还是吞噬作用的调节剂。我们研究了外源性细胞可渗透的 C(6)-神经酰胺是否增强枯否细胞（KCs）的吞噬活性，并影响细胞神经酰胺的水平。使用 Percoll 系统，通过胶原酶消化和差速离心从大鼠中分离 KCs。通过将 KCs 与荧光结合的乳胶珠孵育后，通过流式细胞术分析测量吞噬活性，并通过液相色谱串联质谱法（LC-MS/MS）分析细胞神经酰胺的水平。在这项研究中，我们发现可渗透的 C(6)-神经酰胺通过一种鞘氨醇再循环途径增加内源性神经酰胺的细胞水平，从而增强 KCs 的吞噬作用。
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