Balraj Pauline, Sidek Huraizah, Suppiah Jeyanthi, Khoo Alan Soo Beng, Saat Zainah
Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia.
Malays J Pathol. 2011 Jun;33(1):7-12.
The 2009 pandemic influenza A(H1N1) was first detected in Malaysia in May 2009. It quickly spread in the general population and contributed to a number of influenza-like illness. The objective of the study is to characterize genetic changes in early Malaysian isolates of mild and severe illness of the novel influenza, and to compare sequences of viruses circulating in Malaysia to those in other countries between May to September 2009. Viral isolates of 56 mild cases and 10 severe (intensive care unit or fatal) cases were sequenced for haemagglutinin (HA) and neuraminidase (NA). Genome sequencing of the viral RNA was conducted on 5 isolates (3 were from fatal cases). Highly conserved sequences with few sporadic variations were identified in HA and NA. E374K and D222N were identified in 2 viral isolates from patients with severe illness. Phylogenetic analysis showed close genetic relatedness to the vaccine strain A/California/07/09 and other isolates circulating worldwide during the same period. Sporadic variations were identified in the viral isolates, however a larger sample size is required to make associations with disease severity.
2009年甲型H1N1流感大流行于2009年5月首次在马来西亚被发现。它迅速在普通人群中传播，并导致了一些流感样疾病。本研究的目的是描述马来西亚早期新型流感轻症和重症病例分离株的基因变化，并比较2009年5月至9月期间在马来西亚流行的病毒序列与其他国家的病毒序列。对56例轻症病例和10例重症（重症监护病房或死亡）病例的病毒分离株进行了血凝素（HA）和神经氨酸酶（NA）测序。对5株分离株（3株来自死亡病例）进行了病毒RNA的基因组测序。在HA和NA中鉴定出高度保守的序列，仅有少数散在变异。在2例重症患者的病毒分离株中鉴定出E374K和D222N。系统发育分析表明，其与疫苗株A/California/07/09以及同期在全球流行的其他分离株具有密切的遗传相关性。在病毒分离株中发现了散在变异，然而需要更大的样本量才能将其与疾病严重程度联系起来。
