• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Methionine sulfoximine resistance in Mycobacterium tuberculosis is due to a single nucleotide deletion resulting in increased expression of the major glutamine synthetase, GlnA1.结核分枝杆菌中亚精氨酸琥珀酸亚胺抗性是由于单个核苷酸缺失导致主要谷氨酰胺合成酶 GlnA1 表达增加所致。
Microb Drug Resist. 2011 Sep;17(3):351-5. doi: 10.1089/mdr.2010.0125.
2
All four Mycobacterium tuberculosis glnA genes encode glutamine synthetase activities but only GlnA1 is abundantly expressed and essential for bacterial homeostasis.结核分枝杆菌的所有四个谷氨酰胺合成酶基因（glnA）均编码谷氨酰胺合成酶活性，但只有谷氨酰胺合成酶1（GlnA1）大量表达，且对细菌的内环境稳定至关重要。
Mol Microbiol. 2005 Nov;58(4):1157-72. doi: 10.1111/j.1365-2958.2005.04899.x.
3
An inhibitor of exported Mycobacterium tuberculosis glutamine synthetase selectively blocks the growth of pathogenic mycobacteria in axenic culture and in human monocytes: extracellular proteins as potential novel drug targets.结核分枝杆菌分泌型谷氨酰胺合成酶抑制剂可选择性地阻断无菌培养及人单核细胞中致病性分枝杆菌的生长：细胞外蛋白作为潜在的新型药物靶点。
J Exp Med. 1999 May 3;189(9):1425-36. doi: 10.1084/jem.189.9.1425.
4
Glutamine synthetase sequence evolution in the mycobacteria and their use as molecular markers for Actinobacteria speciation.分枝杆菌中谷氨酰胺合成酶序列的进化及其作为放线菌物种形成分子标记的应用。
BMC Evol Biol. 2009 Feb 26;9:48. doi: 10.1186/1471-2148-9-48.
5
Functional analysis of GlnE, an essential adenylyl transferase in Mycobacterium tuberculosis.结核分枝杆菌中必需的腺苷酰转移酶GlnE的功能分析
J Bacteriol. 2008 Jul;190(14):4894-902. doi: 10.1128/JB.00166-08. Epub 2008 May 9.
6
Independent transcription of glutamine synthetase (glnA2) and glutamine synthetase adenylyltransferase (glnE) in Mycobacterium bovis and Mycobacterium tuberculosis.牛分枝杆菌和结核分枝杆菌中谷氨酰胺合成酶（glnA2）和谷氨酰胺合成酶腺苷酰转移酶（glnE）的独立转录
Tuberculosis (Edinb). 2008 Sep;88(5):382-9. doi: 10.1016/j.tube.2008.02.006. Epub 2008 Apr 28.
7
Inhibition of Mycobacterium tuberculosis glutamine synthetase as a novel antibiotic strategy against tuberculosis: demonstration of efficacy in vivo.抑制结核分枝杆菌谷氨酰胺合成酶作为一种抗结核的新型抗生素策略：体内疗效证明
Infect Immun. 2003 Jan;71(1):456-64. doi: 10.1128/IAI.71.1.456-464.2003.
8
High extracellular levels of Mycobacterium tuberculosis glutamine synthetase and superoxide dismutase in actively growing cultures are due to high expression and extracellular stability rather than to a protein-specific export mechanism.在活跃生长的培养物中，结核分枝杆菌谷氨酰胺合成酶和超氧化物歧化酶的细胞外水平较高，这是由于高表达和细胞外稳定性，而非蛋白质特异性输出机制所致。
Infect Immun. 2001 Oct;69(10):6348-63. doi: 10.1128/IAI.69.10.6348-6363.2001.
9
Effect of the glutamine synthetase inhibitor, methionine sulfoximine, on the growth and differentiation of Dictyostelium discoideum.谷氨酰胺合成酶抑制剂甲硫氨酸亚砜亚胺对盘基网柄菌生长和分化的影响。
FEMS Microbiol Lett. 1997 Jun 15;151(2):163-8. doi: 10.1111/j.1574-6968.1997.tb12565.x.
10
Treatment of Mycobacterium tuberculosis with antisense oligonucleotides to glutamine synthetase mRNA inhibits glutamine synthetase activity, formation of the poly-L-glutamate/glutamine cell wall structure, and bacterial replication.用谷氨酰胺合成酶mRNA的反义寡核苷酸治疗结核分枝杆菌可抑制谷氨酰胺合成酶活性、多聚-L-谷氨酸/谷氨酰胺细胞壁结构的形成以及细菌复制。
Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):418-23. doi: 10.1073/pnas.97.1.418.

引用本文的文献

1
Glutamine metabolism inhibition has dual immunomodulatory and antibacterial activities against Mycobacterium tuberculosis.谷氨酰胺代谢抑制对结核分枝杆菌具有双重免疫调节和抗菌活性。
Nat Commun. 2023 Nov 16;14(1):7427. doi: 10.1038/s41467-023-43304-0.
2
Nitrogen metabolism in mycobacteria: the key genes and targeted antimicrobials.分枝杆菌中的氮代谢：关键基因与靶向抗菌药物
Front Microbiol. 2023 May 18;14:1149041. doi: 10.3389/fmicb.2023.1149041. eCollection 2023.
3
Human Kinase IGF1R/IR Inhibitor Linsitinib Controls the In Vitro and Intracellular Growth of .人源激酶 IGF1R/IR 抑制剂林替司汀抑制. 的体外和细胞内生长。
ACS Infect Dis. 2022 Oct 14;8(10):2019-2027. doi: 10.1021/acsinfecdis.2c00278. Epub 2022 Sep 1.
4
Mechanistic Basis for ATP-Dependent Inhibition of Glutamine Synthetase by Tabtoxinine-β-lactam.烟草毒素-β-内酰胺对谷氨酰胺合成酶ATP依赖性抑制的机制基础
Biochemistry. 2018 Jan 9;57(1):117-135. doi: 10.1021/acs.biochem.7b00838. Epub 2017 Oct 31.
5
In Salmonella enterica, the Gcn5-related acetyltransferase MddA (formerly YncA) acetylates methionine sulfoximine and methionine sulfone, blocking their toxic effects.在沙门氏菌中，Gcn5 相关乙酰转移酶 MddA（以前称为 YncA）乙酰化甲硫氨酸亚砜和甲硫氨酸砜，阻断它们的毒性作用。
J Bacteriol. 2015 Jan;197(2):314-25. doi: 10.1128/JB.02311-14. Epub 2014 Nov 3.
6
Inhibition of glutamine synthetase: a potential drug target in Mycobacterium tuberculosis.谷氨酰胺合成酶的抑制作用：结核分枝杆菌中的一个潜在药物靶点。
Molecules. 2014 Aug 26;19(9):13161-76. doi: 10.3390/molecules190913161.
7
Inhibition of human glutamine synthetase by L-methionine-S,R-sulfoximine-relevance to the treatment of neurological diseases.L-蛋氨酸-S，R-亚砜亚胺对人谷氨酰胺合成酶的抑制作用——与神经疾病治疗的相关性
Metab Brain Dis. 2014 Dec;29(4):983-9. doi: 10.1007/s11011-013-9439-6. Epub 2013 Oct 18.

本文引用的文献

1
Use of DNA arrays to study transcriptional responses to antimycobacterial compounds.利用DNA阵列研究对抗分枝杆菌化合物的转录反应。
Methods Mol Biol. 2010;642:75-91. doi: 10.1007/978-1-60327-279-7_6.
2
The heat shock response of Mycobacterium tuberculosis: linking gene expression, immunology and pathogenesis.结核分枝杆菌的热休克反应：连接基因表达、免疫学与发病机制
Comp Funct Genomics. 2002;3(4):348-51. doi: 10.1002/cfg.183.
3
Functional analysis of GlnE, an essential adenylyl transferase in Mycobacterium tuberculosis.结核分枝杆菌中必需的腺苷酰转移酶GlnE的功能分析
J Bacteriol. 2008 Jul;190(14):4894-902. doi: 10.1128/JB.00166-08. Epub 2008 May 9.
4
Protection elicited by two glutamine auxotrophs of Mycobacterium tuberculosis and in vivo growth phenotypes of the four unique glutamine synthetase mutants in a murine model.结核分枝杆菌的两种谷氨酰胺营养缺陷型所引发的保护作用以及四种独特谷氨酰胺合成酶突变体在小鼠模型中的体内生长表型。
Infect Immun. 2006 Nov;74(11):6491-5. doi: 10.1128/IAI.00531-06.
5
All four Mycobacterium tuberculosis glnA genes encode glutamine synthetase activities but only GlnA1 is abundantly expressed and essential for bacterial homeostasis.结核分枝杆菌的所有四个谷氨酰胺合成酶基因（glnA）均编码谷氨酰胺合成酶活性，但只有谷氨酰胺合成酶1（GlnA1）大量表达，且对细菌的内环境稳定至关重要。
Mol Microbiol. 2005 Nov;58(4):1157-72. doi: 10.1111/j.1365-2958.2005.04899.x.
6
The use of microarray analysis to determine the gene expression profiles of Mycobacterium tuberculosis in response to anti-bacterial compounds.利用微阵列分析确定结核分枝杆菌对抗菌化合物反应的基因表达谱。
Tuberculosis (Edinb). 2004;84(3-4):263-74. doi: 10.1016/j.tube.2003.12.005.
7
Dissection of the heat-shock response in Mycobacterium tuberculosis using mutants and microarrays.利用突变体和微阵列剖析结核分枝杆菌中的热休克反应。
Microbiology (Reading). 2002 Oct;148(Pt 10):3129-3138. doi: 10.1099/00221287-148-10-3129.
8
glnE is an essential gene in Mycobacterium tuberculosis.谷氨酰胺合成酶基因（glnE）是结核分枝杆菌中的一个必需基因。
J Bacteriol. 2000 Oct;182(20):5715-20. doi: 10.1128/JB.182.20.5715-5720.2000.
9
An inhibitor of exported Mycobacterium tuberculosis glutamine synthetase selectively blocks the growth of pathogenic mycobacteria in axenic culture and in human monocytes: extracellular proteins as potential novel drug targets.结核分枝杆菌分泌型谷氨酰胺合成酶抑制剂可选择性地阻断无菌培养及人单核细胞中致病性分枝杆菌的生长：细胞外蛋白作为潜在的新型药物靶点。
J Exp Med. 1999 May 3;189(9):1425-36. doi: 10.1084/jem.189.9.1425.
10
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.从全基因组序列解读结核分枝杆菌的生物学特性。
Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.

结核分枝杆菌中亚精氨酸琥珀酸亚胺抗性是由于单个核苷酸缺失导致主要谷氨酰胺合成酶 GlnA1 表达增加所致。

Methionine sulfoximine resistance in Mycobacterium tuberculosis is due to a single nucleotide deletion resulting in increased expression of the major glutamine synthetase, GlnA1.

机构信息

Barts & The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom.

出版信息

Microb Drug Resist. 2011 Sep;17(3):351-5. doi: 10.1089/mdr.2010.0125.

DOI:10.1089/mdr.2010.0125
PMID:21875360
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3161625/
Abstract

We investigated the effect of methionine sulfoximine (MetSox), a potent inhibitor of glutamine synthetase, on Mycobacterium tuberculosis. M. tuberculosis encodes four glutamine synthetases, of which MetSox targets the type I enzyme encoded by glnA1. Transcriptional profiling revealed that glutamate synthetase (gltB) and a type II glutamine synthetase (glnA3) were induced after exposure to MetSox. In addition, we observed a high rate (10(-5)) of spontaneous resistance to MetSox. All resistant strains had a single-nucleotide deletion in the 5' region of glnA1, and Western analysis revealed that GlnA1 expression was increased in resistant as compared with sensitive strains. These data show that M. tuberculosis can respond to the effect of MetSox inhibition either by up-regulation of GlnA3 or by GlnA1. The high frequency of resistance suggests that MetSox and other compounds specifically targeting GlnA1 are not likely to become successful anti-mycobacterial agents.

摘要

我们研究了蛋氨酸亚砜（MetSox）对结核分枝杆菌的影响。结核分枝杆菌编码四种谷氨酰胺合成酶，MetSox 靶向 glnA1 编码的 I 型酶。转录谱分析显示，暴露于 MetSox 后谷氨酸合成酶（gltB）和 II 型谷氨酰胺合成酶（glnA3）被诱导。此外，我们观察到对 MetSox 的自发耐药率很高（10(-5)）。所有耐药株在 glnA1 的 5'区域都有一个单核苷酸缺失，Western 分析显示耐药株中 GlnA1 的表达高于敏感株。这些数据表明，结核分枝杆菌可以通过上调 GlnA3 或 GlnA1 来应对 MetSox 抑制的影响。高耐药率表明 MetSox 和其他专门针对 GlnA1 的化合物不太可能成为成功的抗分枝杆菌药物。