[赖氨酰氧化酶对人胃癌HGC-27细胞体外迁移和黏附的影响]

[Effect of lysyl oxidase on migration and adhesion of human gastric cancer HGC-27 cells in vitro].

作者信息

Ma Bai-jing, Huang Ling, Han Mei, Wang Yu-qiao, Zhao Jian-ning, Sun Bo-jian, Lin Jia-jing, Song Ting-ge

机构信息

Department of Pathogenic Biology and Immunology, Ningxia Medical University, Yinchuan 750004, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2011 May;33(5):331-3.

PMID:21875459

Abstract

OBJECTIVE

To study the effects of lysyl oxidase (LOX) on the migration and adhesion of the human gastric cancer cell line HGC-27 cells in vitro.

METHODS

The human gastric cancer cell line HGC-27 cells were cultured in vitro, and treated with different concentration of β-aminopropionitrile (BAPN). The ability of migration was assessed by wound-healing assay. The ability of adhesion was detected by homogenous and heterogeneous adhesion experiments.

RESULTS

Compared that with 0 mmol/L BAPN, the ability of migration of the cells after treatment with 0.2 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was increased from (6.97 ± 0.07) × 10(3)/ml to (7.78 ± 0.11) × 10(3)/ml; and the number of cells with heterogeneous adhesion was decreased from (8.98 ± 0.15) × 10(3)/ml to (8.35 ± 0.10) × 10(3)/ml, both < 0.05. Compared with that of cells treated with 0 mmol/L and 0.2 mmol/L BAPN, the migration ability of cells after treatment with 0.3 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was raised to (8.02 ± 0.11) × 10(3)/ml and the number of cells with heterogeneous adhesion was down to (7.93 ± 0.07) × 10(3)/ml (P < 0.05).

CONCLUSION

LOX may promote the metastasis of cancer cells by enhancing invasion, increasing heterogeneous adhesion and decreasing homogeneous adhesion.

摘要

目的

研究赖氨酰氧化酶（LOX）对人胃癌细胞系HGC - 27细胞体外迁移和黏附的影响。

方法

体外培养人胃癌细胞系HGC - 27细胞，并用不同浓度的β - 氨基丙腈（BAPN）处理。通过伤口愈合试验评估迁移能力。通过同质和异质黏附实验检测黏附能力。

结果

与0 mmol/L BAPN相比，0.2 mmol/L BAPN处理后的细胞在8、24、32和48 h的迁移能力下降；同质黏附的细胞数量从（6.97 ± 0.07）× 10³/ml增加到（7.78 ± 0.11）× 10³/ml；异质黏附的细胞数量从（8.98 ± 0.15）× 10³/ml减少到（8.35 ± 0.10）× 10³/ml，两者均P < 0.05。与0 mmol/L和0.2 mmol/L BAPN处理的细胞相比，0.3 mmol/L BAPN处理后的细胞在8、24、32和48 h的迁移能力下降；同质黏附的细胞数量增加到（8.02 ± 0.11）× 10³/ml，异质黏附的细胞数量下降到（7.93 ± 0.07）× 10³/ml（P < 0.05）。