对氯苯丙氨酸增加大鼠杏仁核中谷氨酸受体1的转录。
P-chlorophenylalanine increases glutamate receptor 1 transcription in rat amygdala.
作者信息
Tran Lee, Keele N Bradley
机构信息
Institute for Biomedical Studies, Baylor University, Waco, Texas, USA.
出版信息
Neuroreport. 2011 Oct 26;22(15):758-61. doi: 10.1097/WNR.0b013e32834ae2a1.
Abstract
The amygdala is a key limbic structure strongly implicated in both epilepsy and anxiety disorders. Epilepsy-like mechanisms involve an increased glutamatergic activity, whereas disturbances in serotonin [5-hydroxytryptamine (5-HT)] systems are associated with anxiety-like behavior. Previous studies suggest that low 5-HT increases amygdala excitability, but the molecular mechanisms are not well characterized. Herein we explore the ability of low serotonin to increase glutamate receptor transcription. Using quantitative reverse transcriptase-polymerase chain reaction, we found that rats treated with P-chlorophenylalanine, an inhibitor of tyrosine-5-hydroxylase, resulted in a 21-fold increase in glutamate receptor 1 (GluR1) mRNA expression in the amygdala. These results suggest that low 5-HT induces hyperexcitability of amygdala neurons by increasing GluR1 transcription, and the upregulation of amygdala GluR1 may be important in the pathophysiology of anxiety disorders.
摘要
杏仁核是一个关键的边缘系统结构，与癫痫和焦虑症都密切相关。癫痫样机制涉及谷氨酸能活性增加，而血清素[5-羟色胺（5-HT）]系统紊乱与焦虑样行为有关。先前的研究表明，低5-HT会增加杏仁核的兴奋性，但其分子机制尚未得到充分表征。在此，我们探讨了低血清素增加谷氨酸受体转录的能力。使用定量逆转录聚合酶链反应，我们发现用酪氨酸-5-羟化酶抑制剂对氯苯丙氨酸处理的大鼠，其杏仁核中谷氨酸受体1（GluR1）mRNA表达增加了21倍。这些结果表明，低5-HT通过增加GluR1转录诱导杏仁核神经元的过度兴奋，杏仁核GluR1的上调可能在焦虑症的病理生理学中起重要作用。
相似文献
1
P-chlorophenylalanine increases glutamate receptor 1 transcription in rat amygdala.对氯苯丙氨酸增加大鼠杏仁核中谷氨酸受体1的转录。
Neuroreport. 2011 Oct 26;22(15):758-61. doi: 10.1097/WNR.0b013e32834ae2a1.
2
Phenytoin normalizes exaggerated fear behavior in p-chlorophenylalanine (PCPA)-treated rats.苯妥英可使对氯苯丙氨酸（PCPA）处理的大鼠过度恐惧行为恢复正常。
Epilepsy Behav. 2006 Dec;9(4):557-63. doi: 10.1016/j.yebeh.2006.09.002. Epub 2006 Oct 12.
3
Depletion of serotonin in the basolateral amygdala elevates glutamate receptors and facilitates fear-potentiated startle.基底外侧杏仁核中 5-羟色胺的耗竭会增加谷氨酸受体并促进恐惧增强的惊跳反应。
Transl Psychiatry. 2013 Sep 3;3(9):e298. doi: 10.1038/tp.2013.66.
4
In utero exposure to serotonergic drugs alters neonatal expression of 5-HT(1A) receptor transcripts: a quantitative RT-PCR study.子宫内暴露于血清素能药物会改变5-羟色胺（1A）受体转录物的新生儿表达：一项定量逆转录聚合酶链反应研究。
Int J Dev Neurosci. 2000 Apr-Jun;18(2-3):171-6. doi: 10.1016/s0736-5748(99)00085-4.
5
Involvement of serotonergic pathways in mediating the neuronal activity and genetic transcription of neuroendocrine corticotropin-releasing factor in the brain of systemically endotoxin-challenged rats.血清素能通路参与介导全身内毒素攻击大鼠脑内神经内分泌促肾上腺皮质激素释放因子的神经元活动和基因转录。
Neuroscience. 1999 Jan;88(1):223-40. doi: 10.1016/s0306-4522(98)00369-8.
6
[Changes of central AMPA receptor subunits and related protein mRNA expression in immobilization stressed rats and effect of Xiaoyaosan on them].[束缚应激大鼠中枢AMPA受体亚基及相关蛋白mRNA表达的变化及逍遥散对其的影响]
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Dec;27(12):1110-5.
7
Decreased AMPA GluR2, but not GluR3, mRNA expression in rat amygdala and dorsal hippocampus following morphine-induced behavioural sensitization.吗啡诱导行为敏化后，大鼠杏仁核和背侧海马中AMPA GluR2而非GluR3的mRNA表达降低。
Clin Exp Pharmacol Physiol. 2008 Nov;35(11):1321-30. doi: 10.1111/j.1440-1681.2008.05004.x. Epub 2008 Jul 29.
8
CaMKIIα knockdown decreases anxiety in the open field and low serotonin-induced upregulation of GluA1 in the basolateral amygdala.钙/钙调蛋白依赖性蛋白激酶IIα（CaMKIIα）基因敲低可减轻旷场实验中的焦虑，并降低血清素水平低所诱导的基底外侧杏仁核中谷氨酸受体1（GluA1）的上调。
Behav Brain Res. 2016 Apr 15;303:152-9. doi: 10.1016/j.bbr.2016.01.053. Epub 2016 Jan 26.
9
Preprotachykinin and preproenkephalin mRNA expression within striatal subregions in response to altered serotonin transmission.前速激肽原和前脑啡肽原mRNA在纹状体亚区域内的表达对5-羟色胺传递改变的反应。
Brain Res. 1996 Sep 2;732(1-2):25-35. doi: 10.1016/0006-8993(96)00483-0.
10
Chronic ethanol ingestion modulates proanxiety factors expressed in rat central amygdala.长期摄入乙醇会调节大鼠中央杏仁核中表达的促焦虑因子。
Alcohol. 2005 Jun;36(2):83-90. doi: 10.1016/j.alcohol.2005.07.004.
引用本文的文献
1
Specific behaviors during auditory fear conditioning and postsynaptic expression of AMPA receptors in the basolateral amygdala predict interindividual differences in fear generalization in male rats.在听觉恐惧条件反射期间的特定行为和外侧杏仁核中 AMPA 受体的突触后表达可预测雄性大鼠中恐惧泛化的个体间差异。
Learn Mem. 2023 Apr 14;30(4):74-84. doi: 10.1101/lm.053612.122. Print 2023 Apr.
2
Anxiety and Gene Expression Enhancement in Mice Exposed to Glyphosate-Based Herbicide.接触草甘膦基除草剂的小鼠的焦虑与基因表达增强
Toxics. 2022 Apr 29;10(5):226. doi: 10.3390/toxics10050226.
3
Deep Brain Stimulation Modified Autism-Like Deficits via the Serotonin System in a Valproic Acid-Induced Rat Model.深部脑刺激通过丙戊酸诱导的大鼠模型中的血清素系统改善自闭症样缺陷。
Int J Mol Sci. 2018 Sep 19;19(9):2840. doi: 10.3390/ijms19092840.
4
CaMKIIα knockdown decreases anxiety in the open field and low serotonin-induced upregulation of GluA1 in the basolateral amygdala.钙/钙调蛋白依赖性蛋白激酶IIα（CaMKIIα）基因敲低可减轻旷场实验中的焦虑，并降低血清素水平低所诱导的基底外侧杏仁核中谷氨酸受体1（GluA1）的上调。
Behav Brain Res. 2016 Apr 15;303:152-9. doi: 10.1016/j.bbr.2016.01.053. Epub 2016 Jan 26.
5
BAG3 facilitates the clearance of endogenous tau in primary neurons.BAG3促进原代神经元中内源性tau蛋白的清除。
Neurobiol Aging. 2015 Jan;36(1):241-8. doi: 10.1016/j.neurobiolaging.2014.08.012. Epub 2014 Aug 16.
6
Depletion of serotonin in the basolateral amygdala elevates glutamate receptors and facilitates fear-potentiated startle.基底外侧杏仁核中 5-羟色胺的耗竭会增加谷氨酸受体并促进恐惧增强的惊跳反应。
Transl Psychiatry. 2013 Sep 3;3(9):e298. doi: 10.1038/tp.2013.66.
7
Age-associated remodeling of the intestinal epithelial barrier.年龄相关的肠道上皮屏障重构。
J Gerontol A Biol Sci Med Sci. 2013 Sep;68(9):1045-56. doi: 10.1093/gerona/glt106. Epub 2013 Jul 20.
8
Time-dependent cross talk between spinal serotonin 5-HT2A receptor and mGluR1 subserves spinal hyperexcitability and neuropathic pain after nerve injury.时间依赖性的脊髓 5-羟色胺 2A 受体和 mGluR1 之间的串扰是神经损伤后脊髓过度兴奋和神经性疼痛的基础。
J Neurosci. 2012 Sep 26;32(39):13568-81. doi: 10.1523/JNEUROSCI.1364-12.2012.
9
The involvement of noradrenaline in rapid eye movement sleep mentation.去甲肾上腺素在快速眼动睡眠思维中的作用。
Front Neurol. 2011 Dec 12;2:81. doi: 10.3389/fneur.2011.00081. eCollection 2011.
本文引用的文献
1
Stress impairs 5-HT2A receptor-mediated serotonergic facilitation of GABA release in juvenile rat basolateral amygdala.应激会损害幼鼠基底外侧杏仁核中5-HT2A受体介导的5-羟色胺促进γ-氨基丁酸释放的作用。
Neuropsychopharmacology. 2009 Jan;34(2):410-23. doi: 10.1038/npp.2008.71. Epub 2008 Jun 4.
2
Kindling and sensitization as models for affective episode recurrence, cyclicity, and tolerance phenomena.点燃和致敏作为情感发作复发、周期性和耐受现象的模型。
Neurosci Biobehav Rev. 2007;31(6):858-73. doi: 10.1016/j.neubiorev.2007.04.003. Epub 2007 Apr 24.
3
Phenytoin normalizes exaggerated fear behavior in p-chlorophenylalanine (PCPA)-treated rats.苯妥英可使对氯苯丙氨酸（PCPA）处理的大鼠过度恐惧行为恢复正常。
Epilepsy Behav. 2006 Dec;9(4):557-63. doi: 10.1016/j.yebeh.2006.09.002. Epub 2006 Oct 12.
4
5-HT1A and 5-HT1B receptor agonists and aggression: a pharmacological challenge of the serotonin deficiency hypothesis.5-羟色胺1A和5-羟色胺1B受体激动剂与攻击性：血清素缺乏假说的药理学挑战
Eur J Pharmacol. 2005 Dec 5;526(1-3):125-39. doi: 10.1016/j.ejphar.2005.09.065. Epub 2005 Nov 28.
5
Kindling-induced changes in plasticity of the rat amygdala and hippocampus.点燃诱导的大鼠杏仁核和海马可塑性变化。
Learn Mem. 2005 Sep-Oct;12(5):520-6. doi: 10.1101/lm.4205.
6
The role of serotonin in impulsive and aggressive behaviors associated with epilepsy-like neuronal hyperexcitability in the amygdala.
Epilepsy Behav. 2005 Nov;7(3):325-35. doi: 10.1016/j.yebeh.2005.06.014. Epub 2005 Aug 15.
7
Postsynaptic receptor trafficking underlying a form of associative learning.一种联合学习形式背后的突触后受体运输
Science. 2005 Apr 1;308(5718):83-8. doi: 10.1126/science.1103944. Epub 2005 Mar 3.
8
Anxiolytic effects of kindling role of anatomical location of the kindling electrode in response to kindling of the right basolateral amygdala.点燃电极的解剖位置在右侧基底外侧杏仁核点燃反应中的抗焦虑作用。
Brain Res. 2004 Oct 22;1024(1-2):44-58. doi: 10.1016/j.brainres.2004.06.074.
9
The neurobiology of antiepileptic drugs for the treatment of nonepileptic conditions.用于治疗非癫痫性疾病的抗癫痫药物的神经生物学。
Nat Med. 2004 Jul;10(7):685-92. doi: 10.1038/nm1074.
10
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.使用实时定量PCR和2(-ΔΔC(T))方法分析相对基因表达数据。
Methods. 2001 Dec;25(4):402-8. doi: 10.1006/meth.2001.1262.
Zotero 插件