College of Resource and Environment, Shaanxi University of Science and Technology, Xi'an 710021, China.
Environ Technol. 2011 Apr;32(5-6):663-72. doi: 10.1080/09593330.2010.510534.
Microorganism communities and chemical characteristics in sludge-bamboo charcoal composting system were investigated to find the effect of bamboo charcoal on composting. According to a plate count test, abundances of bacteria, fungi and actinomycetes in the treatment with bamboo charcoal were several times higher than those in treatment without bamboo charcoal. In addition, terminal restriction fragment length polymorphism (TRFLP) analysis indicated that the bacterial community diversity in treatment with bamboo charcoal was greater than that of the control. Both results demonstrated that amendment with bamboo charcoal can increase microorganism population and microorganism community diversity in a sludge composting system. Moreover, the results of FTIR spectroscopy disclosed that aerobic composting can promote the formation of surface acid groups on bamboo charcoal. These surface acid groups may deprotonate and react with NH4+ to form stable complexes. Therefore, the increase of functional groups accompanied with greater assimilation of nitrogen by microorganisms could reduce nitrogen loss in sludge composting.
研究了污泥-竹炭堆肥系统中的微生物群落和化学特性，以探究竹炭对堆肥的影响。根据平板计数试验，添加竹炭的处理中细菌、真菌和放线菌的丰度比无竹炭处理高几倍。此外，末端限制性片段长度多态性（TRFLP）分析表明，添加竹炭的处理中细菌群落多样性大于对照。这两个结果都表明，在污泥堆肥系统中添加竹炭可以增加微生物种群和微生物群落的多样性。此外，傅里叶变换红外光谱（FTIR）的结果表明，好氧堆肥可以促进竹炭表面酸性基团的形成。这些表面酸性基团可能会去质子化并与 NH4+反应形成稳定的配合物。因此，功能基团的增加伴随着微生物对氮的更大吸收，可减少污泥堆肥中的氮损失。
