Macdonald Catriona A, Campbell Colin D, Bacon Jeffrey R, Singh Brajesh K
Macaulay Institute, Craigiebuckler, Aberdeen, UK.
FEMS Microbiol Ecol. 2008 Sep;65(3):555-64. doi: 10.1111/j.1574-6941.2008.00538.x.
The long-term impacts of Cu- and Zn-rich sewage sludge additions on the structure of the microbial community in a field under pasture were investigated using a combination of multiplex-terminal restriction fragment length polymorphism (M-TRFLP) and T-RFLP profiling approaches. Changes in the community structure of bacteria, fungi, archaea and actinobacteria were observed in soils that had previously received Cu- (50-200 mg kg(-1) soil) and Zn- (150-450 mg kg(-1) soil) rich sewage sludge additions. Changes in the structure of all microbial groups measured were observed at Cu and Zn rates below the current EU guidelines (135 mg kg(-1) Cu and 300 mg kg(-1) Zn). The response of the fungal community, and to a lesser extent the bacterial and archaeal community, to Cu was dose dependent. The fungal community also showed a dose-dependent response to Zn, which was not observed in the other microbial groups assessed. Redundancy analysis demonstrated that individual terminal restriction fragments responded to both Cu and Zn and these may have potential as genetic markers of long-term metal effects in soil.
采用多重末端限制性片段长度多态性（M-TRFLP）和T-RFLP分析方法相结合的方式，研究了添加富含铜和锌的污水污泥对牧场土壤中微生物群落结构的长期影响。在先前添加过富含铜（50-200毫克/千克土壤）和锌（150-450毫克/千克土壤）的污水污泥的土壤中，观察到细菌、真菌、古菌和放线菌的群落结构发生了变化。在低于当前欧盟指导标准（135毫克/千克铜和300毫克/千克锌）的铜和锌添加量下，所测量的所有微生物类群的结构都发生了变化。真菌群落以及在较小程度上细菌和古菌群落对铜的反应呈剂量依赖性。真菌群落对锌也表现出剂量依赖性反应，而在所评估的其他微生物类群中未观察到这种反应。冗余分析表明，单个末端限制性片段对铜和锌都有反应，这些片段可能有潜力作为土壤中长期金属效应的遗传标记。
