Department of Chemistry, Faculty of Engineering, and the Center for Nanoscale Science and Engineering, University of Georgia, Athens, Georgia 30602, USA.
Langmuir. 2011 Oct 4;27(19):12253-60. doi: 10.1021/la202344w. Epub 2011 Sep 12.
In this article, a methacrylate-based spiropyran-containing copolymer was used as a colorimetric sensor to identify multiple metal ions simultaneously. Through UV-vis absorption spectroscopy, the relative binding affinity of merocyanine to each metal ion was investigated by displacement studies of a bound metal ion with a second metal ion of a higher binding affinity. We also show that because each metal ion gives rise to a distinct spectral response, partial least-squares discriminant analysis (PLS-DA) can be used to analyze the UV-vis absorbance spectra to identify the two metal ions that are present in solution at varying concentrations simply by dipping a coated polymer substrate into solution after irradiation. Partial least-squares regression analysis (PLS) was used to determine the metal ions in solution for several binary mixtures quantitatively. We also demonstrate that the quantitative determination depends on the relative binding preference of merocyanine to each metal ion.
在本文中，使用一种基于甲基丙烯酸酯的含螺吡喃的共聚物作为比色传感器，以同时识别多种金属离子。通过紫外-可见吸收光谱，通过用具有更高结合亲和力的第二金属离子置换结合的金属离子，研究了金属络合的变色体与每种金属离子的相对结合亲和力。我们还表明，由于每种金属离子都会产生独特的光谱响应，因此可以使用偏最小二乘判别分析（PLS-DA）来分析紫外-可见吸收光谱，只需在辐照后将涂覆的聚合物基底浸入溶液中，即可识别出溶液中存在的两种金属离子以不同浓度。偏最小二乘回归分析（PLS）用于定量测定几种二元混合物中的金属离子。我们还证明，定量测定取决于变色体对每种金属离子的相对结合偏好。
