Sasidharan A, Gnanam A
School of Biological Sciences, Madurai Kamaraj University, India.
Indian J Biochem Biophys. 1990 Feb;27(1):1-4.
The alteration of photosynthetic membrane proteins in relation to the disappearance of pigments during the heterotrophic growth of Chlorella protothecoides was investigated. Chlorophylls and certain polypeptides associated with the LHC II disappeared after 50 hr of heterotrophic growth but the 24 kDa apoprotein constituting LHC II was not affected. Immunological analysis indicated that the chlorophylls and the light harvesting complex proteins of the thylakoid membranes are not tightly coupled and the latter is retained in its native form irrespective of the presence or absence of the former. The circumstantial evidence that the other photosynthetic membrane polypeptides are degraded along with the pigments due to increased proteolytic activity in the rapidly dividing heterotrophic cells indicate that chlorophyll synthesis is not a pre-requisite for the synthesis of the LHC II apoprotein.
研究了原球藻异养生长过程中光合膜蛋白的变化与色素消失的关系。叶绿素和与光系统II捕光复合体（LHC II）相关的某些多肽在异养生长50小时后消失，但构成LHC II的24 kDa脱辅基蛋白不受影响。免疫分析表明，类囊体膜中的叶绿素和光捕获复合蛋白并非紧密偶联，无论前者是否存在，后者都以天然形式保留。间接证据表明，由于快速分裂的异养细胞中蛋白水解活性增加，其他光合膜多肽与色素一起被降解，这表明叶绿素合成不是LHC II脱辅基蛋白合成的先决条件。
