Tanaka Y, Tozawa H, Koyanagi Y, Shida H
Department of Immunology, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
J Immunol. 1990 Jun 1;144(11):4202-11.
We established rat T cell lines expressing human T cell leukemia virus type I (HTLV-I) Ag from inbred strains of rats, WKA/H, DA, and F344, to study CTL response against the HTLV-I-infected cells. HTLV-I-specific Ag expressed in these rat cells were HTLV-I gag Ag, p19, p24, and p15, and pX Ag, p40tax and p27rex, but not env Ag, as determined by immunofluorescence and immunoblot assays. By immunization of rats with syngeneic HTLV-I-infected cells, CTL against syngeneic HTLV-I-infected cells and antibodies to HTLV-I Ag were generated in WKA/H and DA rats. The bulk CTL cultures from WKA/H and DA rats lysed specifically syngeneic SV40-transformed kidney cells infected with recombinant vaccinia viruses (RVV) expressing HTLV-I gag and pX Ag, but not those infected with RVV expressing HTLV-I env Ag or a control vaccinia virus. From WKA/H rat CTL cultures, four CTL clones reactive with syngeneic HTLV-I-infected cells were isolated, three of which were specific for p27rex/p21x, but the Ag recognized by the other CTL clone was not defined with any RVV used. These results indicate that HTLV-I gag and pX gene products are recognized by MHC-restricted rat CTL specific for syngeneic HTLV-I-infected cells.
我们从近交系大鼠WKA/H、DA和F344建立了表达人I型T细胞白血病病毒（HTLV-I）抗原的大鼠T细胞系，以研究针对HTLV-I感染细胞的CTL反应。通过免疫荧光和免疫印迹分析确定，这些大鼠细胞中表达的HTLV-I特异性抗原为HTLV-I gag抗原、p19、p24和p15，以及pX抗原、p40tax和p27rex，但不包括env抗原。用同基因HTLV-I感染细胞免疫大鼠后，WKA/H和DA大鼠产生了针对同基因HTLV-I感染细胞的CTL和针对HTLV-I抗原的抗体。来自WKA/H和DA大鼠的大量CTL培养物特异性裂解了感染表达HTLV-I gag和pX抗原的重组痘苗病毒（RVV）的同基因SV40转化肾细胞，但未裂解感染表达HTLV-I env抗原的RVV或对照痘苗病毒的细胞。从WKA/H大鼠CTL培养物中分离出四个与同基因HTLV-I感染细胞反应的CTL克隆，其中三个对p27rex/p21x具有特异性，但另一个CTL克隆识别的抗原与所用的任何RVV均未明确。这些结果表明，HTLV-I gag和pX基因产物可被针对同基因HTLV-I感染细胞的MHC限制性大鼠CTL识别。
