• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酵母KRE5基因编码一种可能参与（1→6）-β-D-葡聚糖合成及正常细胞生长所需的内质网蛋白。

The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (1----6)-beta-D-glucan synthesis and normal cell growth.

作者信息

Meaden P, Hill K, Wagner J, Slipetz D, Sommer S S, Bussey H

机构信息

Department of Biology, McGill University, Montreal, Quebec, Canada.

出版信息

Mol Cell Biol. 1990 Jun;10(6):3013-9. doi: 10.1128/mcb.10.6.3013-3019.1990.

DOI:10.1128/mcb.10.6.3013-3019.1990
PMID:2188106
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360665/
Abstract

Yeast kre mutants define a pathway of cell wall (1----6)-beta-D-glucan synthesis, and mutants in genes KRE5 and KRE6 appear to interact early in such a pathway. We have cloned KRE5, and the sequence predicts the product to be a large, hydrophilic, secretory glycoprotein which contains the COOH-terminal endoplasmic reticulum retention signal, HDEL. Deletion of the KRE5 gene resulted in cells with aberrant morphology and extremely compromised growth. Suppressors to the KRE5 deletions arose at a frequency of 1 in 10(7) to 1 in 10(8) and permitted an analysis of deletions which were found to contain no alkali-insoluble (1----6)-beta-D-glucan. These results indicate a role for (1----6)-beta-D-glucan in normal cell growth and suggest a model for sequential assembly of (1----6)-beta-D-glucan in the yeast secretory pathway.

摘要

酵母kre突变体定义了一条细胞壁（1→6）-β-D-葡聚糖合成途径，KRE5和KRE6基因的突变体似乎在此途径的早期相互作用。我们克隆了KRE5，其序列预测产物是一种大型亲水性分泌糖蛋白，含有COOH末端内质网滞留信号HDEL。KRE5基因的缺失导致细胞形态异常且生长严重受损。KRE5缺失的抑制子出现频率为10^7分之一到10^8分之一，并允许对缺失进行分析，发现这些缺失不包含碱不溶性（1→6）-β-D-葡聚糖。这些结果表明（1→6）-β-D-葡聚糖在正常细胞生长中起作用，并提出了酵母分泌途径中（1→6）-β-D-葡聚糖顺序组装的模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c565/360665/89f62ef669a2/molcellb00042-0588-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c565/360665/6d7232151d2b/molcellb00042-0587-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c565/360665/89f62ef669a2/molcellb00042-0588-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c565/360665/6d7232151d2b/molcellb00042-0587-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c565/360665/89f62ef669a2/molcellb00042-0588-a.jpg

相似文献

1
The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (1----6)-beta-D-glucan synthesis and normal cell growth.酵母KRE5基因编码一种可能参与（1→6）-β-D-葡聚糖合成及正常细胞生长所需的内质网蛋白。
Mol Cell Biol. 1990 Jun;10(6):3013-9. doi: 10.1128/mcb.10.6.3013-3019.1990.
2
Characterization of the yeast (1-->6)-beta-glucan biosynthetic components, Kre6p and Skn1p, and genetic interactions between the PKC1 pathway and extracellular matrix assembly.酵母（1→6）-β-葡聚糖生物合成成分Kre6p和Skn1p的特性，以及PKC1途径与细胞外基质组装之间的遗传相互作用。
J Cell Biol. 1994 Oct;127(2):567-79. doi: 10.1083/jcb.127.2.567.
3
Saccharomyces cerevisiae Big1p, a putative endoplasmic reticulum membrane protein required for normal levels of cell wall beta-1,6-glucan.酿酒酵母Big1p，一种细胞壁β-1,6-葡聚糖正常水平所需的假定内质网膜蛋白。
Yeast. 2002 Jun 30;19(9):783-93. doi: 10.1002/yea.873.
4
The yeast KRE9 gene encodes an O glycoprotein involved in cell surface beta-glucan assembly.酵母KRE9基因编码一种参与细胞表面β-葡聚糖组装的O-糖蛋白。
Mol Cell Biol. 1993 Oct;13(10):6346-56. doi: 10.1128/mcb.13.10.6346-6356.1993.
5
Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly.酵母KRE基因提供了细胞壁β-葡聚糖组装途径的证据。
J Cell Biol. 1990 May;110(5):1833-43. doi: 10.1083/jcb.110.5.1833.
6
CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta 1,6-glucan assembly.CWH41编码一种参与β-1,6-葡聚糖组装的新型内质网膜N-糖蛋白。
J Bacteriol. 1996 Feb;178(4):1162-71. doi: 10.1128/jb.178.4.1162-1171.1996.
7
A mutational analysis of killer toxin resistance in Saccharomyces cerevisiae identifies new genes involved in cell wall (1-->6)-beta-glucan synthesis.酿酒酵母中杀伤毒素抗性的突变分析鉴定出参与细胞壁（1→6）-β-葡聚糖合成的新基因。
Genetics. 1993 Apr;133(4):837-49. doi: 10.1093/genetics/133.4.837.
8
Yeast beta-glucan synthesis: KRE6 encodes a predicted type II membrane protein required for glucan synthesis in vivo and for glucan synthase activity in vitro.酵母β-葡聚糖合成：KRE6编码一种预测的II型膜蛋白，该蛋白在体内葡聚糖合成以及体外葡聚糖合酶活性方面是必需的。
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11295-9. doi: 10.1073/pnas.88.24.11295.
9
Genetic, biochemical, and morphological evidence for the involvement of N-glycosylation in biosynthesis of the cell wall beta1,6-glucan of Saccharomyces cerevisiae.N-糖基化参与酿酒酵母细胞壁β1,6-葡聚糖生物合成的遗传学、生物化学及形态学证据。
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15381-6. doi: 10.1073/pnas.2536561100. Epub 2003 Dec 15.
10
Isolation of the Candida albicans homologs of Saccharomyces cerevisiae KRE6 and SKN1: expression and physiological function.酿酒酵母KRE6和SKN1的白色念珠菌同源物的分离：表达及生理功能
J Bacteriol. 1997 Apr;179(7):2363-72. doi: 10.1128/jb.179.7.2363-2372.1997.

引用本文的文献

1
Production of yeast cell wall polysaccharides-β-glucan and chitin by using food waste substrates: Biosynthesis, production, extraction, and purification methods.利用食品废弃物底物生产酵母细胞壁多糖-β-葡聚糖和几丁质：生物合成、生产、提取及纯化方法
Compr Rev Food Sci Food Saf. 2025 May;24(3):e70161. doi: 10.1111/1541-4337.70161.
2
The Viral K1 Killer Yeast System: Toxicity, Immunity, and Resistance.病毒K1杀伤酵母系统：毒性、免疫与抗性
Yeast. 2024 Nov;41(11-12):668-680. doi: 10.1002/yea.3987. Epub 2025 Jan 24.
3
Unlocking the genome of the non-sourdough Kazachstania humilis MAW1: insights into inhibitory factors and phenotypic properties.

本文引用的文献

1
Improved method for determination of plasma polysaccharides with tryptophan.用色氨酸测定血浆多糖的改进方法。
Proc Soc Exp Biol Med. 1953 Nov;84(2):289-91.
2
Yeast killer plasmid mutations affecting toxin secretion and activity and toxin immunity function.影响毒素分泌、活性及毒素免疫功能的酵母杀伤质粒突变
Mol Cell Biol. 1982 Apr;2(4):346-54. doi: 10.1128/mcb.2.4.346-354.1982.
3
How signal sequences maintain cleavage specificity.信号序列如何维持切割特异性。
解锁非发酵面团哈萨克斯坦酒香酵母 MAW1 的基因组：对抑制因子和表型特性的认识。
Microb Cell Fact. 2024 Apr 15;23(1):111. doi: 10.1186/s12934-024-02380-7.
4
Identifying genetic variants associated with amphotericin B (AMB) resistance in via merbased GWAS.通过基于标记的全基因组关联研究（GWAS）鉴定与两性霉素B（AMB）耐药性相关的基因变异。
Front Genet. 2023 May 9;14:1133593. doi: 10.3389/fgene.2023.1133593. eCollection 2023.
5
Involvement of the Cell Wall Integrity Pathway of Saccharomyces cerevisiae in Protection against Cadmium and Arsenate Stresses.酿酒酵母细胞壁完整性途径在抵抗镉和砷酸盐胁迫中的作用。
Appl Environ Microbiol. 2020 Oct 15;86(21). doi: 10.1128/AEM.01339-20.
6
Origin and Evolution of Two Independently Duplicated Genes Encoding UDP- Glucose: Glycoprotein Glucosyltransferases in and Vertebrates.和脊椎动物中编码 UDP-葡萄糖：糖蛋白葡萄糖基转移酶的两个独立复制基因的起源和进化。
G3 (Bethesda). 2020 Feb 6;10(2):755-768. doi: 10.1534/g3.119.400868.
7
Transcriptome analysis of Valsa mali reveals its response mechanism to the biocontrol actinomycete Saccharothrix yanglingensis Hhs.015.转录组分析揭示了苹果腐烂病菌对生防放线菌杨凌链霉菌 Hhs.015 的响应机制。
BMC Microbiol. 2018 Aug 22;18(1):90. doi: 10.1186/s12866-018-1225-5.
8
Characterization of aromatic residue-controlled protein retention in the endoplasmic reticulum of .内质网中芳香族残基控制的蛋白质滞留的表征。 （注：原文句末的“of.”表述不完整，这里按完整语义翻译）
J Biol Chem. 2017 Dec 15;292(50):20707-20719. doi: 10.1074/jbc.M117.812107. Epub 2017 Oct 16.
9
The Dual Activity Responsible for the Elongation and Branching of β-(1,3)-Glucan in the Fungal Cell Wall.负责真菌细胞壁中β-(1,3)-葡聚糖伸长和分支的双重活性。
mBio. 2017 Jun 20;8(3):e00619-17. doi: 10.1128/mBio.00619-17.
10
β-(1,3)-Glucan Unmasking in Some Candida albicans Mutants Correlates with Increases in Cell Wall Surface Roughness and Decreases in Cell Wall Elasticity.一些白色念珠菌突变体中β-(1,3)-葡聚糖的暴露与细胞壁表面粗糙度增加和细胞壁弹性降低相关。
Infect Immun. 2016 Dec 29;85(1). doi: 10.1128/IAI.00601-16. Print 2017 Jan.
J Mol Biol. 1984 Feb 25;173(2):243-51. doi: 10.1016/0022-2836(84)90192-x.
4
Analysis of wall glucans from yeast, hyphal and germ-tube forming cells of Candida albicans.白色念珠菌酵母细胞、菌丝细胞和芽管形成细胞中壁葡聚糖的分析。
J Gen Microbiol. 1984 Dec;130(12):3295-301. doi: 10.1099/00221287-130-12-3295.
5
Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN.酿酒酵母中的超级杀手突变抑制了L-A-HN对M2双链RNA的排斥，并在存在M和L-A-HN的情况下赋予冷敏感性。
Mol Cell Biol. 1984 Apr;4(4):761-70. doi: 10.1128/mcb.4.4.761-770.1984.
6
One-step gene disruption in yeast.酵母中的一步基因破坏
Methods Enzymol. 1983;101:202-11. doi: 10.1016/0076-6879(83)01015-0.
7
Cell wall receptor for yeast killer toxin: involvement of (1 leads to 6)-beta-D-glucan.酵母杀伤毒素的细胞壁受体：（1→6）-β-D-葡聚糖的参与
J Bacteriol. 1983 Apr;154(1):161-9. doi: 10.1128/jb.154.1.161-169.1983.
8
A system for shotgun DNA sequencing.一种用于鸟枪法DNA测序的系统。
Nucleic Acids Res. 1981 Jan 24;9(2):309-21. doi: 10.1093/nar/9.2.309.
9
Rapid DNA isolations for enzymatic and hybridization analysis.用于酶促和杂交分析的快速DNA分离
Methods Enzymol. 1980;65(1):404-11. doi: 10.1016/s0076-6879(80)65051-4.
10
The structure of a beta-(1--6)-D-glucan from yeast cell walls.来自酵母细胞壁的β-（1,6）-D-葡聚糖的结构
Biochem J. 1973 Sep;135(1):31-6. doi: 10.1042/bj1350031.