从巴西里约热内卢市一所大学医院无菌临床标本中分离出的非结核分枝杆菌的鉴定。
Identification of nontuberculous mycobacteria isolated from clinical sterile sites in patients at a university hospital in the city of Rio de Janeiro, Brazil.
机构信息
Federal University of Rio de Janeiro – Rio de Janeiro, Brazil.
出版信息
J Bras Pneumol. 2011 Jul-Aug;37(4):521-6. doi: 10.1590/s1806-37132011000400015.
OBJECTIVE
To identify nontuberculous mycobacteria (NTM) isolated from sterile sites in patients hospitalized between 2001 and 2006 at the Clementino Fraga Filho University Hospital, located in the city of Rio de Janeiro, Brazil.
METHODS
During the study period, 34 NTM isolates from sterile sites of 14 patients, most of whom were HIV-positive, were submitted to phenotypic identification and hsp65 PCR-restriction enzyme analysis (PRA).
RESULTS
Most isolates were identified as Mycobacterium avium, followed by M. monacense, M. kansasii, and M. abscessus.
CONCLUSIONS
The combination of PRA, a relatively simple and inexpensive method, with the evaluation of a few phenotypic characteristics can allow NTM to be accurately identified in the routine of clinical laboratories.
目的
鉴定 2001 年至 2006 年间在巴西里约热内卢克莱门蒂诺·弗雷加·菲略大学医院住院的无菌部位分离出的非结核分枝杆菌（NTM）。
方法
在研究期间，对来自 14 名患者的 34 株无菌部位的 NTM 分离株进行了表型鉴定和 hsp65 PCR-限制性内切酶分析（PRA）。
结果
大多数分离株被鉴定为鸟分枝杆菌，其次是 M. monacense、M. kansasii 和 M. abscessus。
结论
PRA 是一种相对简单且廉价的方法，结合少数表型特征的评估，可以使临床实验室常规准确地鉴定 NTM。
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