Department of Geriatric Pharmacology and Therapeutics, Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
Rheumatol Int. 2012 Oct;32(10):3309-13. doi: 10.1007/s00296-011-2069-6. Epub 2011 Sep 1.
The aim of this study is to compare the expression level of histamine H(4) receptor (H(4)R) mRNA in synovial tissues of rheumatoid arthritis (RA) and osteoarthritis (OA) patients, and to study correlation of results with clinical characteristics of patients with RA. Synovial tissues were obtained from 7 RA and 7 OA patients undergoing artificial arthroplasty. Serum levels of erythrocyte sedimentation rate, C-reactive protein, matrix metalloproteinase-3 (MMP-3), rheumatoid factors, and cyclic citrullinated peptide antibodies were determined. The expression of H(4)R mRNA in synovial tissues was determined by real-time polymerase chain reaction. Expression of H(1)R and H(4)R mRNA were significantly lower in RA compared with OA patients (P < 0.005), while expression of H(2)R mRNA was comparable in both. While a significant negative correlation was found between H(4)R expression and serum MMP-3 concentration (r = -0.70, P < 0.05), no correlation was found between MMP-3 and H(1)R (r = -0.52) or H(2)R (r = 0.23). This study supports the supposition that H(4)R in synovial tissue may play a role in cartilage and bone destruction by influencing the secretion of MMP-3 in patients with RA.
本研究旨在比较类风湿关节炎（RA）和骨关节炎（OA）患者滑膜组织中组氨酸 H（4）受体（H（4）R）mRNA 的表达水平，并研究其与 RA 患者临床特征的相关性。我们从 7 例接受人工关节置换术的 RA 和 7 例 OA 患者的滑膜组织中获取组织。检测红细胞沉降率、C 反应蛋白、基质金属蛋白酶-3（MMP-3）、类风湿因子和环瓜氨酸肽抗体的血清水平。通过实时聚合酶链反应检测滑膜组织中 H（4）R mRNA 的表达。RA 患者滑膜组织中 H（1）R 和 H（4）R mRNA 的表达明显低于 OA 患者（P < 0.005），而 H（2）R mRNA 的表达在两者之间无差异。H（4）R 表达与血清 MMP-3 浓度呈显著负相关（r = -0.70，P < 0.05），而 MMP-3 与 H（1）R（r = -0.52）或 H（2）R（r = 0.23）之间无相关性。本研究支持这样的假设，即滑膜组织中的 H（4）R 通过影响 MMP-3 的分泌，可能在 RA 患者的软骨和骨破坏中发挥作用。
