Department of Molecular Cell Biology, SBRI, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
Mol Cell Biochem. 2012 Jan;360(1-2):47-50. doi: 10.1007/s11010-011-1042-8. Epub 2011 Sep 1.
Interactions between various signaling pathways enable a fine control of cellular activities. When the cells are subjected to activation of TGF-β signaling and PKC signaling, PKC phosphorylation of Smad3 abrogates binding and transcriptional activity of Smad3 leading to suppression of TGF-β response. We studied this interaction between Smads and PKC in different cell types to examine cell specificity of the interaction. We found that the outcome of the interaction between Smads and PKC depends on cell types and inducibility of a regulatory molecule Tsc-22. In this report, we showed that induced Tsc-22 leads to enhancement of TGF-β-dependent signaling and the enhancement was blocked by expression of a dominant-negative Tsc-22 mutant. Its effect on cellular differentiation was also examined.
各种信号通路之间的相互作用可以精细地控制细胞的活动。当细胞受到 TGF-β 信号和 PKC 信号的激活时，PKC 对 Smad3 的磷酸化会破坏 Smad3 的结合和转录活性，从而抑制 TGF-β 反应。我们在不同的细胞类型中研究了 Smads 和 PKC 之间的这种相互作用，以检查相互作用的细胞特异性。我们发现，Smads 和 PKC 之间的相互作用的结果取决于细胞类型和调节分子 Tsc-22 的诱导性。在本报告中，我们表明，诱导的 Tsc-22 导致 TGF-β 依赖性信号的增强，而这种增强被表达显性负性 Tsc-22 突变体所阻断。还检查了它对细胞分化的影响。
