[Expression of IL-18, IL-6 and oxidative stress in rat peritoneal mesothelial cells stimulated by lipopolysaccharide].

AIM

To observe the expression of IL-18, IL-6 and the production of oxidative stress Malondialdehyde (MDA) in rat peritoneal mesothelial cells (RPMC) stimulated by lipopolysaccharide (LPS).

METHODS

RPMC were primordially culture and stimulated with different concentrations (1, 10, 100 mg/L) LPS for 6h; RPMC were stimulated by 10 mg/L LPS for 3, 6, 12, 24 h. IL-18 mRNA was detected by real time-PCR. IL-6 and IL-18 were detected by ELISA in supernatants. MDA was measured by thiobarbiuric acid method.

RESULTS

Compared with the control group, the expression of IL-18, IL-6 and MDA were gradually increased by LPS with different concentrations (P < 0.05) ; the expression of targets above were little by little increased with stimulated time prolonging. then the peak of IL-18 were appeared in 12 h.

CONCLUSION

The expression of proinflammatory cytokine IL-6 and the production of oxidative stress MDA was increased in the RPMCs stimulated by LPS, then leads to the perpetuating amplificated inflammation, aggravate peritoneal impairment and hyperfiltration losed.