[松属素对脑线粒体呼吸功能的影响]
[Effect of pinocembrin on brain mitochondrial respiratory function].
作者信息
Shi Li-Li, Qiang Gui-Fen, Gao Mei, Zhang Heng-Ai, Chen Bai-Nian, Yu Xiao-Yan, Xuan Zhao-Hong, Wang Qiao-Yun, Du Guan-Hua
机构信息
National Center for Pharmaceutical Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
出版信息
Yao Xue Xue Bao. 2011 Jun;46(6):642-9.
There are growing evidences that pinocembrin has better neuroprotective effect. In the present study, the effect of pinocembrin on mitochondrial respiratory function was evaluated in global brain ischemia/ reperfusion (4-vessel occlusion, 4-VO) rats. The results showed that pinocembrin improved the respiratory activity of 4-VO brain mitochondria, through increasing ADP/O, state 3 respiration state (V3), respiration control rate index (RCI) and oxidative phosphorylation rate (OPR). And then, the effect of pinocembrin on brain mitochondria was verified in vitro. The results showed that pinocembrin increased ADP/O, state 3 respiration state, respiration control rate index, oxidative phosphorylation rate in NADH/FADH2 dependent respiratory chain and decreased state 4 respiration state (V4) in NADH dependent respiratory chain. Pinocembrin improved ATP content in brain mitochondria in vitro and in SH-SY5Y cells.
越来越多的证据表明，松属素具有更好的神经保护作用。在本研究中，在全脑缺血/再灌注（四动脉闭塞，4-VO）大鼠中评估了松属素对线粒体呼吸功能的影响。结果表明，松属素通过增加ADP/O、状态3呼吸状态（V3）、呼吸控制率指数（RCI）和氧化磷酸化率（OPR），改善了4-VO脑线粒体的呼吸活性。然后，在体外验证了松属素对脑线粒体的作用。结果表明，松属素增加了NADH/FADH2依赖性呼吸链中的ADP/O、状态3呼吸状态、呼吸控制率指数、氧化磷酸化率，并降低了NADH依赖性呼吸链中的状态4呼吸状态（V4）。松属素在体外和SH-SY5Y细胞中提高了脑线粒体中的ATP含量。
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