Bakhidze E V, Lavrinovich O E, Chepik O F, Kiselev O I
Vopr Onkol. 2011;57(3):318-21.
Our study included 112 patients with squamous cell cervical carcinoma la-lib stages (FIGO). All of them were restaged on the basis of histological evidence after surgical treatment. Ninety-eight were staged at final analysis. Human papilloma virus (HPV) was detected in endocervical smears and paraffin blocks of lymph nodes by PCR and real-time PCR. Oncogenic HPV in primary tumor was detected in 86 (87.8%); two or more genotypes--65 (75.58%): still more--21 (24.4%). In the latter group, the frequency of regional lymph node metastases was significantly higher. HPV DNA was identified in iliac lymph nodes in 29 (26.6%); 27 of them had metastases to those nodes. HPV in DNA which was significantly more frequent in involved lymphocytes may be used as marker. Since our method for early detection of metastases to regional lymph nodes is highly specific, it may be recommended as a diagnostic procedure.
我们的研究纳入了112例FIGO分期为Ⅰa-Ⅰb期的宫颈鳞状细胞癌患者。所有患者均在手术治疗后根据组织学证据进行重新分期。最终分析时98例完成分期。通过PCR和实时PCR检测宫颈涂片和淋巴结石蜡块中的人乳头瘤病毒（HPV）。原发肿瘤中检测到致癌性HPV的有86例（87.8%）；两种或更多基因型的有65例（75.58%）；更多基因型的有21例（24.4%）。在后一组中，区域淋巴结转移的频率显著更高。在29例（26.6%）的髂淋巴结中鉴定出HPV DNA；其中27例有这些淋巴结转移。在受累淋巴细胞中明显更常见的DNA中的HPV可作为标志物。由于我们早期检测区域淋巴结转移的方法具有高度特异性，可推荐作为一种诊断程序。
