Kumari Vibha, Rathore Gaurav, Chauhan U K, Pandey A K, Lakra W S
National Bureau of Fish Genetic Resources, Canal Ring Road, Lucknow - 226 002, India.
J Environ Biol. 2011 Mar;32(2):153-9.
Seasonal changes in abundance of nitrifiers (ammonia-oxidizing and nitrite-oxidizing bacteria) in surface and bottom water of freshwater ponds were examined with respect to temperature, DO, pH as well as concentration of ammonia and nitrite. The most probable number (MPN) of ammonia-oxidizers in different ponds varied from 1297 +/- 3.6 to 1673.23 +/- 0.36 ml(-1) in bottom and 720.5 +/- 8.1 to 955.3 +/- 10.8 ml(-1) in surface water during the rainy season while the MPN ranged from 1074 +/- 1.07 to 1372.17 +/- 4.6 ml(-1) in bottom and 515 +/- 10.1 to 678 +/- 11.8 ml(-1) in surface water in winter. However, the MPN were greatly reduced in summer and ranged from 435.05 +/- 15.7 to 547.54 +/- 2.12 ml(-1) in bottom and 218.7 +/- 7.3 to 368.4 +/- 9.32 ml(-1) in surface water. Similar seasonal trends were also observed in MPN of nitrite-oxidizers. Among all the physico-chemical parameters, abundance of nitrifiers was more positively correlated with ammonia and nitrite concentration in all the seasons. The abundance of nitrifiers in surface and bottom water was highest in rainy season followed by winter and modest in summer. The potential nitrification activities and oxidation rates were shown to be linear and activity of ammonia-oxidizing and nitrite-oxidizing bacteria was highest during rainy season.
针对温度、溶解氧（DO）、pH值以及氨和亚硝酸盐浓度，研究了淡水池塘表层水和底层水中硝化细菌（氨氧化细菌和亚硝酸盐氧化细菌）数量的季节性变化。雨季期间，不同池塘中氨氧化细菌的最大可能数（MPN）在底层水中为1297±3.6至1673.23±0.36 ml⁻¹，在表层水中为720.5±8.1至955.3±10.8 ml⁻¹；冬季时，底层水中MPN为1074±1.07至1372.17±4.6 ml⁻¹，表层水中为515±10.1至678±11.8 ml⁻¹。然而，夏季MPN大幅降低；底层水中为435.05±15.7至547.54±2.12 ml⁻¹，表层水中为218.7±7.3至368.4±9.32 ml⁻¹。亚硝酸盐氧化细菌的MPN也呈现出类似的季节性趋势。在所有理化参数中，硝化细菌数量在所有季节与氨和亚硝酸盐浓度的正相关性更强。表层水和底层水中硝化细菌数量在雨季最高，其次是冬季，夏季则适中。潜在硝化活性和氧化速率呈线性关系，氨氧化细菌和亚硝酸盐氧化细菌的活性在雨季最高。
