Pesticide Science Department and Plant Protection College of Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Insects, Ministry of Agriculture, Nanjing 210095, China.
J Econ Entomol. 2011 Aug;104(4):1364-8. doi: 10.1603/EC11074.
Cross-resistance to two fipronil analogs, butene-fipronil and ethiprole, was detected in fipronil-resistant field populations and a resistant laboratory strain of the planthopper Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), although the two analogs have not been used widely in rice-growing areas in China. The results showed that six field populations with 23.8-43.3-fold resistance to fipronil had reached a higher level of cross-resistance to ethiprole (resistance ratio [RR] = 47.1-100.9-fold) and had a minor level of cross-resistance (RR = 3.4- 8.1-fold) to butene-fipronil. After 10 generations of selection, the RR to fipronil increased from 7.3-fold to 41.3-fold. At the same time, the insect increased cross-RR to ethiprole from 16.3-fold to 65.6-fold, whereas it had only minor increase in cross-resistance to butene-fipronil from 2.8-fold to 4.0-fold. These results confirmed that fipronil-resistant N. lugens could develop a higher level of cross-resistance to ethiprole, although it still maintained a lower level cross-resistance to butene-fipronil. Our data suggest that ethiprole is not a suitable alternative for controlling N. lugens, once the insect has developed a high level resistance to fipronil. Further investigation is necessary to understand the cross-resistance mechanisms in N. lugens.
尽管两种类似物在我国水稻种植区尚未广泛使用，但在田间种群和抗药性实验室品系褐飞虱（Nilaparvata lugens）（半翅目：飞虱科）中已检测到对两种拟除虫菊酯类杀虫剂氟虫腈（fipronil）类似物，烯虫酯（butene-fipronil）和乙虫腈（ethiprole）的交叉抗性。结果表明，6 个田间种群对氟虫腈的抗性为 23.8-43.3 倍，对乙虫腈的抗性达到了更高水平（抗性比 [RR] = 47.1-100.9 倍），对烯虫酯的抗性为中等水平（RR = 3.4-8.1 倍）。经过 10 代选择，对氟虫腈的 RR 从 7.3 倍增加到 41.3 倍。同时，昆虫对乙虫腈的交叉 RR 从 16.3 倍增加到 65.6 倍，而对烯虫酯的交叉抗性仅略有增加，从 2.8 倍增加到 4.0 倍。这些结果证实，氟虫腈抗性褐飞虱可以对乙虫腈产生更高水平的交叉抗性，尽管它对烯虫酯仍保持较低水平的交叉抗性。我们的数据表明，一旦昆虫对氟虫腈产生高水平抗性，乙虫腈就不适合作为防治褐飞虱的替代药剂。需要进一步研究以了解褐飞虱的交叉抗性机制。
