Department of Materials Science and Engineering and Institute for Biomedical Research, Korea University, Anam-dong, Seongbuk-gu, Seoul, Republic of Korea, 136-713.
Anal Chem. 2011 Oct 1;83(19):7364-70. doi: 10.1021/ac201291y. Epub 2011 Sep 1.
We have investigated the hybridization properties of DNA-gold nanoparticle conjugates and have discovered that the hybridization properties are dramatically affected by controlling various synthetic and environmental conditions. We have further demonstrated that moderate DNA loading instead of high loading per nanoparticle significantly enhances the hybridization rates of DNA-gold nanoparticle conjugates, which allows one to precisely design their hybridization properties to distinguish a single-nucleotide polymorphism (SNP). A diagnostic application for the colorimetric detection of an SNP associated with a mutation in the breast cancer gene BRCA1 has been carefully designed and demonstrated.
我们研究了 DNA-金纳米粒子缀合物的杂交性质，发现通过控制各种合成和环境条件，可以显著影响杂交性质。我们进一步证明，适度的 DNA 负载而不是每个金纳米粒子的高负载显著提高了 DNA-金纳米粒子缀合物的杂交速率，这使得人们可以精确设计它们的杂交性质，以区分单核苷酸多态性 (SNP)。我们精心设计并展示了一种用于乳腺癌基因 BRCA1 突变相关 SNP 的比色检测的诊断应用。
