Das Chiranjit, Raveendran Jeethu, Bayry Jagadeesh, Rasheed P Abdul
Department of Chemistry, Indian Institute of Technology Palakkad Palakkad Kerala India-678623
Department of Biological Sciences and Engineering, Indian Institute of Technology Palakkad Palakkad Kerala India-678623.
RSC Adv. 2024 Oct 24;14(46):33784-33793. doi: 10.1039/d4ra06191h. eCollection 2024 Oct 23.
We report a simple naked eye colorimetric detection assay developed for the small molecule creatinine using the surface passivation of gold nanoparticles (AuNPs) which is conjugated with a creatinine binding aptamer. The selective binding of creatinine to aptamer sequences causes a decrease in the catalytic activity of AuNPs, and the color change time of the 4-nitrophenol reduction was used for the quantitative colorimetric detection of creatinine. Herein, the surfaces of AuNPs acted as the catalyst for the reduction of 4-nitrophenol (yellow) to 4-aminophenol (colorless), and the passivation with creatinine bound aptamer sequences delayed the reduction. The developed assay was able to detect creatinine in a linear range of 2-20 mM with a limit of detection of 0.87 mM. The developed colorimetric assay was very selective and repeatable and could detect creatinine in the presence of interfering biomolecules. Moreover, the assay showed excellent results for the analysis of creatinine in artificial urine samples. The developed assay can be used as a point of care (POC) device for the naked eye detection of creatinine within few minutes without any instrument support.
我们报道了一种简单的裸眼比色检测方法，该方法利用与肌酐结合适体共轭的金纳米颗粒（AuNPs）的表面钝化作用来检测小分子肌酐。肌酐与适体序列的选择性结合导致AuNPs的催化活性降低，4-硝基苯酚还原反应的颜色变化时间用于肌酐的定量比色检测。在此，AuNPs的表面作为将4-硝基苯酚（黄色）还原为4-氨基苯酚（无色）的催化剂，而与肌酐结合的适体序列进行的钝化作用延迟了还原反应。所开发的检测方法能够在2-20 mM的线性范围内检测肌酐，检测限为0.87 mM。所开发的比色检测方法具有很高的选择性和可重复性，并且能够在存在干扰生物分子的情况下检测肌酐。此外，该检测方法在人工尿液样本中肌酐分析方面显示出优异的结果。所开发的检测方法可作为一种即时检测（POC）设备，在无需任何仪器支持的情况下，几分钟内即可裸眼检测肌酐。
