Akamine Mayumi, Maekawa Kiyoto, Kon Masahiro
School of Environmental Science, The University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan.
Zoolog Sci. 2011 Sep;28(9):652-8. doi: 10.2108/zsj.28.652.
A partial sequence of the mitochondrial cytochrome c oxidase subunit I (COI) gene (745 bp) was determined for 57 specimens of a geotrupid beetle (Phelotrupes auratus) from throughout the Japanese archipelago. Of the 57 beetles examined, 42 haplotypes were identified. Phylogenetic trees inferred using maximum parsimony, neighbor joining, and Bayesian inference methods were highly congruent. Reconstructed phylogenetic relationships indicated that P. auratus from the Japanese archipelago was separated into two distinct lineages: Group A, which consisted of 35 haplotypes from Honshu, Shikoku, and Hokkaido Islands, and Group B, which consisted of seven haplotypes from Kyushu and Yakushima Islands. In addition, two sublineages were also recognized within Group A: Subgroup A-1, which consisted of 11 haplotypes from eastern Honshu and Hokkaido, and Subgroup A-2, which consisted of 10 haplotypes from western Honshu and Shikoku. Average genetic distances within Group A were positively correlated to geographic distance between sampling localities. Phylogenetic relationships among haplotypes did not correspond to subspecies classification.
测定了来自日本列岛各地的57只粪金龟（Phelotrupes auratus）线粒体细胞色素c氧化酶亚基I（COI）基因的部分序列（745 bp）。在检测的57只甲虫中，鉴定出42个单倍型。使用最大简约法、邻接法和贝叶斯推断法推断的系统发育树高度一致。重建的系统发育关系表明，来自日本列岛的P. auratus被分为两个不同的谱系：A组，由来自本州、四国和北海道岛的35个单倍型组成；B组，由来自九州和屋久岛的7个单倍型组成。此外，在A组中还识别出两个亚谱系：A-1亚组，由来自本州东部和北海道的11个单倍型组成；A-2亚组，由来自本州西部和四国的10个单倍型组成。A组内的平均遗传距离与采样地点之间的地理距离呈正相关。单倍型之间的系统发育关系与亚种分类不对应。
