Sakae C, Ishida T
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan.
J Parasitol. 2012 Feb;98(1):224-5. doi: 10.1645/GE-2881.1. Epub 2011 Sep 1.
Toxoplasma gondii infection was studied in 41 Japanese serows ( Capricornis crispus ), a goat-antelope in mainland Japan. Blood and muscle specimens were collected from 41 subjects between 2006 and 2010. Presence of antibodies to T. gondii in the sera was examined by using the latex agglutination test (cutoff titer 1:32); 10 of 41 (24.4%) were seropositive. Toxoplasma gondii DNA was detected in muscle tissue of 1 seropositive serow using a semi-nested PCR assay for the B1 gene. A partial nucleotide sequence (220 bp) corresponding to the B1 gene of T. gondii was obtained by direct sequencing; the sequence was 99.1% identical to that of the RH strain. This study is the first report to show direct evidence for the T. gondii infection in Japanese serows.
在日本本土的一种山羊羚羊——日本鬣羚（Capricornis crispus）中对弓形虫感染情况进行了研究。2006年至2010年间从41只日本鬣羚身上采集了血液和肌肉样本。采用乳胶凝集试验（临界滴度1:32）检测血清中抗弓形虫抗体的存在情况；41只中有10只（24.4%）血清呈阳性。使用针对B1基因的半巢式PCR检测法在1只血清阳性的日本鬣羚的肌肉组织中检测到了弓形虫DNA。通过直接测序获得了与弓形虫B1基因对应的部分核苷酸序列（220 bp）；该序列与RH株的序列有99.1%的同一性。本研究是首次显示日本鬣羚感染弓形虫直接证据的报告。
