Cancer Institute of People's Liberation Army, Xinqiao Hospital, Third Military Medical University, 30 Gootanyan Main Street, Chongqing, P.R.
Cancer Biother Radiopharm. 2011 Oct;26(5):585-92. doi: 10.1089/cbr.2011.0962. Epub 2011 Sep 1.
The objective of the present study was to explore the role of the inhibitor of apoptosis protein (IAP) Livin in radioresistance in nonsmall cell lung cancer (NSCLC).
Lung adenocarcinoma cell lines A549 and SPC-A1 were used for this study. Using the technique of molecular cloning and gene transfection, two Livin isoforms, Livinα and β, respectively, were expressed in A549 cells with the purpose of exploring the role of Livin in radiation resistance of A549 cells. Moreover, a Livin-specific gene-silencing system was developed using SPC-A1 cell line with the purpose of increasing radiosensitivity of SPC-A1 cells.
A549 cells were induced by radiation to express Livin isoforms, Livinα and β. A549 cells expressed Livin isoforms stably after gene transfection and the transfected cells demonstrated characteristics of antiradiation. However, Livin gene-silenced SPC-A1 cells exhibited remarkably enhanced radiation sensitivity.
The IAP Livin is an important molecule in antiradiotherapy of NSCLC. Livin-specific gene silencing is likely to be an effective means to enhance radiation sensitivity of lung cancer.
本研究旨在探讨凋亡抑制蛋白（IAP）Livin 在非小细胞肺癌（NSCLC）放射抵抗中的作用。
本研究采用肺腺癌细胞系 A549 和 SPC-A1。通过分子克隆和基因转染技术，分别在 A549 细胞中表达两种 Livin 异构体 Livinα 和β，旨在探讨 Livin 在 A549 细胞放射抵抗中的作用。此外，利用 SPC-A1 细胞系开发了一种 Livin 特异性基因沉默系统，旨在提高 SPC-A1 细胞的放射敏感性。
A549 细胞经辐射诱导表达 Livin 异构体 Livinα 和β。基因转染后 A549 细胞稳定表达 Livin 异构体，转染细胞表现出抗辐射特性。然而，Livin 基因沉默的 SPC-A1 细胞表现出明显增强的放射敏感性。
IAP Livin 是 NSCLC 放射治疗抵抗的重要分子。Livin 特异性基因沉默可能是提高肺癌放射敏感性的有效手段。
