Lange Christian E, Tobler Kurt, Lehner Angelika, Vetsch Elisabeth, Favrot Claude
Dermatology Department, Clinic for Small Animal Internal Medicine, Institute of Virology and Institute for Food Safety and Hygiene, Vetsuisse Faculty, Zürich, Switzerland.
Vet Dermatol. 2012 Feb;23(1):76-80, e18-9. doi: 10.1111/j.1365-3164.2011.01007.x. Epub 2011 Sep 1.
The seven fully described canine papillomaviruses (CPVs) have been allocated by sequence comparison and other genetic features into three phylogenetic clades. This largely reflects clinical findings, so each sequence of a newly discovered CPV in combination with clinical and pathological details is a valuable piece of evidence. We hypothesize that the genomic sequence of a new CPV can help to predict clinical features and progression, and that this can be tested in subsequent cases. In this case, a 2-year-old female dachshund-mix presented with papillomatosis clinically and histologically characterized as pigmented viral plaques. PCRs using primers evaluated for CPVs successfully amplified papillomavirus (PV) DNA. Sequencing of the products revealed an unknown PV putatively belonging to the PV genus Chi. Rolling circle amplification was used to amplify the entire viral genome. Sequencing revealed a novel PV, designated as CPV8, which was most closely related (63% homology) to the recently discovered CPV4. CPV4 is associated with benign pigmented plaques in pugs. Phylogenetic analysis based on the nucleotide sequences of four viral genes showed that the novel virus was closest to CPV3, CPV4 and CPV5. The presence of viral DNA was confirmed in the lesions by in situ hybridization using specific probes. CPV8 may consequently be regarded as the fourth member of the Chi-papillomavirus genus. All viruses belonging to this genus induce pigmented plaques in dogs. These findings support the hypothesis that genomic sequences can be useful in predicting the clinical features of CPV infection.
通过序列比较和其他遗传特征，已将七种已被充分描述的犬乳头瘤病毒（CPV）划分为三个系统发育分支。这在很大程度上反映了临床发现，因此新发现的CPV的每个序列与临床和病理细节相结合都是一项有价值的证据。我们假设新CPV的基因组序列有助于预测临床特征和病程，并且这可以在后续病例中得到验证。在本病例中，一只2岁的雌性腊肠犬混种犬临床上表现为乳头瘤病，组织学特征为色素沉着性病毒斑块。使用针对CPV评估的引物进行的聚合酶链反应（PCR）成功扩增了乳头瘤病毒（PV）DNA。产物测序显示一种未知的PV，推测属于Chi乳头瘤病毒属。采用滚环扩增法扩增整个病毒基因组。测序显示一种新型PV，命名为CPV8，它与最近发现的CPV4关系最为密切（同源性为63%）。CPV4与哈巴狗的良性色素沉着斑块有关。基于四个病毒基因的核苷酸序列进行的系统发育分析表明，该新型病毒与CPV3、CPV4和CPV5最为接近。使用特异性探针通过原位杂交在病变中证实了病毒DNA的存在。因此，CPV8可能被视为Chi乳头瘤病毒属的第四个成员。该属的所有病毒都会在犬类中诱发色素沉着斑块。这些发现支持了基因组序列可用于预测CPV感染临床特征这一假设。
