Luff J, Rowland P, Mader M, Orr C, Yuan H
North Carolina State University, Raleigh, NC, USA
Histopath Consulting, Worcester, MA, USA.
Vet Pathol. 2016 Nov;53(6):1160-1163. doi: 10.1177/0300985816630795. Epub 2016 Mar 4.
Papillomaviruses (PV) are associated with benign mucosal and cutaneous epithelial proliferations. In dogs, PV-associated pigmented plaques and papillomas can undergo malignant transformation, but this is rare, and most cases of canine squamous cell carcinoma do not arise from PV-induced precursor lesions. We describe herein the progression of pigmented plaques to invasive and metastatic squamous cell carcinoma associated with 2 canine papillomaviruses (CPV) in 2 related Basenji dogs. Immunohistochemistry for PV antigen revealed strong nuclear immunoreactivity within keratinocytes from pigmented plaques from both dogs, consistent with a productive viral infection. Polymerase chain reaction (PCR) using degenerate primers for the L1 gene revealed PV DNA sequences from 2 different CPVs. In situ hybridization for CPV revealed strong hybridization signals within the pigmented plaques and neoplastic squamous epithelial cells from both dogs. We report here progression of PV-associated pigmented plaques to metastatic squamous cell carcinoma within 2 Basenji dogs associated with 2 different CPVs.
乳头瘤病毒（PV）与良性黏膜和皮肤上皮增殖有关。在犬类中，PV相关的色素沉着斑块和乳头状瘤可发生恶性转化，但这种情况很少见，而且大多数犬类鳞状细胞癌并非由PV诱导的前驱病变发展而来。我们在此描述了2只相关巴仙吉犬中与2种犬乳头瘤病毒（CPV）相关的色素沉着斑块发展为侵袭性和转移性鳞状细胞癌的过程。PV抗原的免疫组织化学显示，两只犬色素沉着斑块的角质形成细胞内有强烈的核免疫反应，这与病毒的活跃感染一致。使用针对L1基因的简并引物进行聚合酶链反应（PCR），揭示了来自2种不同CPV的PV DNA序列。CPV的原位杂交显示，两只犬的色素沉着斑块和肿瘤性鳞状上皮细胞内有强烈的杂交信号。我们在此报告了2只巴仙吉犬中与2种不同CPV相关的PV相关色素沉着斑块发展为转移性鳞状细胞癌的过程。
