Orimi Syouji, Mizuno Katsunari, Narahara Michihito, Umakosi Hideko, Kaihara Masanori, Hashimoto Masami
Medical Engineering Center, Onomichi Municipal Hospital, Onimichi, Hiroshima, Japan.
Ther Apher Dial. 2011 Aug;15(4):411-4. doi: 10.1111/j.1744-9987.2011.00973.x.
We examined appropriate flow rates for high collection rates of Total Protein (TP) and Albumin (Alb) and a change of IL-6 concentrations in the cell-free and concentrated ascites reinfusion therapy (CART) process. No significant changes were observed in IL-6 concentration in the ascites storage test and the circulatory stimulation test. However, it was confirmed that the quantity of IL-6 in the ascites decreased by means of filtration and concentration. In some cases, it quickly reached high concentration rates at a higher flow rate (200 mL/min) setting, but clogging or pressure increase has occurred in the hollow fiber filter. Therefore, it was concluded that a low flow rate (50 mL/min) setting was best and provides safer conditions for filtration and concentration in order to collect TP and Alb effectively and reuse it as autologous protein, which is the primary objective of CART.
我们研究了在无细胞浓缩腹水再输注疗法（CART）过程中，实现总蛋白（TP）和白蛋白（Alb）高收集率以及白细胞介素-6（IL-6）浓度变化的合适流速。在腹水储存试验和循环刺激试验中，未观察到IL-6浓度有显著变化。然而，经证实，通过过滤和浓缩，腹水中IL-6的量减少了。在某些情况下，在较高流速（200 mL/分钟）设置下能迅速达到高浓缩率，但中空纤维滤器出现了堵塞或压力增加的情况。因此，得出的结论是，低流速（50 mL/分钟）设置最佳，可为过滤和浓缩提供更安全的条件，以便有效收集TP和Alb并将其作为自体蛋白重复使用，这是CART的主要目标。
