Department of Chemistry, Nanchang University, Nanchang 330031, China.
Anal Biochem. 2011 Dec 15;419(2):76-80. doi: 10.1016/j.ab.2011.08.009. Epub 2011 Aug 12.
The interaction of salbutamol (Sal), an animal growth promoter, with DNA was investigated by differential pulse voltammetry (DPV), cyclic voltammetry (CV), and fluorescence spectroscopy. An irreversible reduction was observed from the cyclic voltammograms, and the reaction mechanism involved a one-electron change irreversible oxidation. In the presence of DNA, the DPV peak current decreased and the Sal peak shifted to higher potentials, indicating that Sal interacted with DNA to form an intercalation Sal-DNA complex. In addition, reaction binding parameters were extracted from the DPV data with the use of the multivariate curve resolution-alternating least squares (MCR-ALS) method; the binding constant and ratio were found to be (2.0±0.5)×10(5) M(-1) and 1:1, respectively. Quantitative voltammetric analysis of Sal was performed in the concentration range of 3.02×10(-6) to 1.23×10(-4) molL(-1), and it was found that the detection limit was 5.11×10(-7) molL(-1) in the presence of 1.00×10(-6) molL(-1) DNA. The method was applied for the determination of Sal in spiked urine and human serum samples, and the calibration was successfully verified.
采用差分脉冲伏安法（DPV）、循环伏安法（CV）和荧光光谱法研究了动物生长促进剂沙丁胺醇（Sal）与 DNA 的相互作用。从循环伏安图中观察到不可逆还原，反应机制涉及单电子变化不可逆氧化。在 DNA 存在下，DPV 峰电流减小，Sal 峰向更高电位移动，表明 Sal 与 DNA 相互作用形成嵌入 Sal-DNA 复合物。此外，使用多元曲线分辨-交替最小二乘法（MCR-ALS）方法从 DPV 数据中提取反应结合参数；结合常数和比值分别为（2.0±0.5）×10（5）M（-1）和 1:1。在存在 1.00×10（-6）molL（-1）DNA 的情况下，对 3.02×10（-6）至 1.23×10（-4）molL（-1）范围内的 Sal 进行了定量伏安分析，发现检测限为 5.11×10（-7）molL（-1）。该方法用于测定加标尿液和人血清样品中的 Sal，并成功验证了校准。
