Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN 55455, United States.
Exp Eye Res. 2011 Nov;93(5):767-70. doi: 10.1016/j.exer.2011.08.010. Epub 2011 Aug 26.
A micro-advancer device that positions a narrow-gauge needle within the vitreous humor of the rat eye is described. The device is compact, simple and inexpensive to manufacture. It consists of an outer guard needle and an inner injection needle that is advanced through the guard needle. With the rat held in a stereotaxic holder and the globe fixed to a stabilizing ring, the outer 25-gauge guard needle is advanced through the sclera using a standard micromanipulator. The inner 31-gauge injection needle is then advanced through the guard needle with a manually controlled leadscrew and carriage mechanism. The inner injection needle is attached to a Hamilton syringe and can be positioned to within microns of the retinal surface under visual observation through a microscope. The injection needle is fixed to the device by a quick-release clamp on the carriage and can be rapidly exchanged while the guard needle remains in place in the vitreous. This permits different solutions to be injected sequentially into the vitreous humor. Recording electrodes, stimulating electrodes, and optical fibers can also be advanced through the guard needle and positioned accurately near the retinal surface or within the retina.
描述了一种将微窄规针头定位在大鼠眼球玻璃体中的微型推进器装置。该装置结构紧凑，制造简单，价格低廉。它由一个外保护针和一个内注射针组成，内注射针通过保护针推进。将大鼠固定在立体定位架上，并将眼球固定在稳定环上，使用标准的微操纵器将 25 号外保护针推进巩膜。然后，通过手动控制的丝杠和滑架机构将 31 号内注射针推进保护针。内注射针连接到 Hamilton 注射器上，并可以通过显微镜在视觉观察下将其定位到视网膜表面的微米范围内。注射针通过滑架上的快速释放夹固定在装置上，并且可以在保护针留在玻璃体中的同时快速更换。这允许将不同的溶液顺序注入玻璃体中。记录电极、刺激电极和光纤也可以通过保护针推进，并准确地定位在视网膜表面附近或视网膜内。
