Molecular Biology Department, Fundación Instituto de Inmunología de Colombia, Bogotá, Colombia.
J Virol Methods. 2011 Dec;178(1-2):68-74. doi: 10.1016/j.jviromet.2011.08.014. Epub 2011 Aug 22.
The aims of this study were to determine the prevalence of HPV infection and evaluate the concordance and performance of two primer sets for detecting single and multiple viral infections. A total of 1810 Colombian women were enrolled in the study, and molecular, cytological and epidemiological analyses were performed. Both concordance and performance of two different PCR amplification primer sets (GP5+/6+ and MY09/11) were assessed. The results showed that 60.2% of females with positive HPV DNA were infected by more than one viral type. The OR for multiple infections was 18.2 when using the MY09/11 primer set and 6.52 with the GP5+/6+ primer set. The results also showed an association between GP5+/6+ positivity and the severity of the disease regarding the cytological findings. It was also found that using a single primer set led to underestimating the prevalence for HPV infection. The simultaneous use of these primer sets is an important tool for the detection of HPV DNA, being equally relevant for identifying multiple infections and low viral DNA copies. This study highlights the importance of suitable assessment of HPV epidemiological profiles; screening programs must also be strengthened to broaden the coverage of the most vulnerable populations.
本研究的目的是确定 HPV 感染的流行率，并评估两种引物检测单一和多重病毒感染的一致性和性能。共纳入 1810 名哥伦比亚女性进行研究，并进行了分子、细胞学和流行病学分析。评估了两种不同的 PCR 扩增引物（GP5+/6+ 和 MY09/11）的一致性和性能。结果表明，60.2%的 HPV DNA 阳性女性感染了一种以上的病毒类型。使用 MY09/11 引物时，多重感染的 OR 为 18.2，使用 GP5+/6+ 引物时为 6.52。结果还显示，GP5+/6+ 阳性与细胞学发现的疾病严重程度之间存在关联。还发现，使用单一引物会导致 HPV 感染的流行率被低估。同时使用这些引物是检测 HPV DNA 的重要工具，对于识别多重感染和低病毒 DNA 拷贝同样重要。本研究强调了对 HPV 流行病学特征进行适当评估的重要性；还必须加强筛查计划，以扩大最脆弱人群的覆盖面。
