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原核生物中“预期”核小体定位模式。

"Anticipated" nucleosome positioning pattern in prokaryotes.

机构信息

Genome Diversity Center, Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel.

出版信息

Gene. 2011 Nov 15;488(1-2):41-5. doi: 10.1016/j.gene.2011.08.002. Epub 2011 Aug 23.

Abstract

Linguistic (word count) analysis of prokaryotic genome sequences, by Shannon N-gram extension, reveals that the dominant hidden motifs in A+T rich genomes are T(A)(T)A and G(A)(T)C with uncertain number of repeating A and T. Since prokaryotic sequences are largely protein-coding, the motifs would correspond to amphipathic alpha-helices with alternating lysine and phenylalanine as preferential polar and non-polar residues. The motifs are also known in eukaryotes, as nucleosome positioning patterns. Their existence in prokaryotes as well may serve for binding of histone-like proteins to DNA. In this case the above patterns in prokaryotes may be considered as "anticipated" nucleosome positioning patterns which, quite likely, existed in prokaryotic genomes before the evolutionary separation between eukaryotes and prokaryotes.

摘要

对原核生物基因组序列进行语言(单词计数)分析,通过香农 N 元扩展,揭示出在富含 A 和 T 的基因组中,主要的隐藏基序是 T(A)(T)A 和 G(A)(T)C,其中 A 和 T 的重复数不确定。由于原核序列主要是蛋白质编码序列,因此这些基序对应于具有交替赖氨酸和苯丙氨酸的两亲性α-螺旋,它们分别是优先的极性和非极性残基。这些基序在真核生物中也被称为核小体定位模式。它们在原核生物中的存在也可能用于组蛋白样蛋白与 DNA 的结合。在这种情况下,原核生物中的上述模式可以被认为是“预期的”核小体定位模式,这些模式很可能在真核生物和原核生物进化分离之前就存在于原核生物基因组中。

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