使用即时检测酶联免疫吸附测定法检测庇护所猫体内针对猫泛白细胞减少症病毒、猫疱疹病毒-1和猫杯状病毒的保护性抗体滴度。
Detection of protective antibody titers against feline panleukopenia virus, feline herpesvirus-1, and feline calicivirus in shelter cats using a point-of-care ELISA.
作者信息
Digangi Brian A, Gray Lauren K, Levy Julie K, Dubovi Edward J, Tucker Sylvia J
机构信息
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA.
出版信息
J Feline Med Surg. 2011 Dec;13(12):912-8. doi: 10.1016/j.jfms.2011.07.009. Epub 2011 Aug 31.
Abstract
Serum antibody titers are a useful measurement of protection against infection (feline panleukopenia virus [FPV]) or clinical disease (feline herpesvirus-1 [FHV] and feline calicivirus [FCV]), and their determination has been recommended as part of disease outbreak management in animal shelters. The objective of this study was to determine the sensitivity, specificity, and inter-observer and inter-assay agreement of two semi-quantitative point-of-care assays for the detection of protective antibody titers (PAT) against FPV, FHV and FCV in shelter cats. Low sensitivity for FPV antibodies (28%) rendered a canine point-of-care assay inappropriate for use in cats. The feline point-of-care assay also had low sensitivity (49%) and low negative predictive value (74%) for FPV PAT detection, but was highly accurate in the assessment of FHV and FCV PAT. Improvements in accuracy and repeatability of FPV PAT determination could make this tool a valuable component of a disease outbreak response in animal shelters.
摘要
血清抗体滴度是衡量针对感染（猫泛白细胞减少症病毒[FPV]）或临床疾病（猫疱疹病毒1型[FHV]和猫杯状病毒[FCV]）的保护作用的有用指标，并且已建议将其测定作为动物收容所疾病爆发管理的一部分。本研究的目的是确定两种半定量即时检测法在检测收容所猫针对FPV、FHV和FCV的保护性抗体滴度（PAT）时的敏感性、特异性以及观察者间和检测间的一致性。犬用即时检测法对FPV抗体的敏感性较低（28%），不适用于猫。猫用即时检测法在检测FPV PAT时也具有较低的敏感性（49%）和较低的阴性预测值（74%），但在评估FHV和FCV PAT方面具有高度准确性。提高FPV PAT测定的准确性和可重复性可使该工具成为动物收容所疾病爆发应对措施中的一个有价值的组成部分。
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3
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4
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