Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, College of Ocean Sciences & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, South Korea.
J Biosci Bioeng. 2011 Dec;112(6):541-4. doi: 10.1016/j.jbiosc.2011.07.023. Epub 2011 Sep 1.
This study, reports the identification and analysis of ferritin M chain, from kelp grouper, Epinephelus bruneus (EbFerM); it comprises 1004 base pair (bp), including 528bp open reading frame (ORF) which encodes 176 amino acid (aa) residues; the calculated molecular weight is 20kDa. The 5'-untranslated region (UTR) possesses 476bp proceeded by a putative Iron Regulatory Element (IRE). Pair wise alignments showed that EbFerM shared 94% identity with that of Larimichthys crocea and Sciaenops. It is expressed in abundance in liver, spleen, and kidney when challenged with Vibrio anguillarum, lipopolysaccharide (LPS), or poly I:C.
本研究报告了褐菖鲉（Epinephelus bruneus）铁蛋白 M 链（EbFerM）的鉴定和分析；EbFerM 由 1004 个碱基对（bp）组成，其中包含 528bp 的开放阅读框（ORF），编码 176 个氨基酸（aa）残基；计算的分子量为 20kDa。5'-非翻译区（UTR）含有 476bp，前面是一个推定的铁调节元件（IRE）。序列比对显示，EbFerM 与大黄鱼和青石斑鱼的同源性为 94%。在受到鳗弧菌、脂多糖（LPS）或聚肌苷酸-聚胞苷酸（poly I:C）刺激时，EbFerM 在肝脏、脾脏和肾脏中大量表达。
