E27 Cellular Biophysics, Technische Universität München, Garching, Germany.
PLoS One. 2011;6(8):e23798. doi: 10.1371/journal.pone.0023798. Epub 2011 Aug 26.
Self organization mechanisms are essential for the cytoskeleton to adapt to the requirements of living cells. They rely on the intricate interplay of cytoskeletal filaments, crosslinking proteins and molecular motors. Here we present an in vitro minimal model system consisting of actin filaments, fascin and myosin-II filaments exhibiting pulsatile collective dynamics and superdiffusive transport properties. Both phenomena rely on the complex competition of crosslinking molecules and motor filaments in the network. They are only observed if the relative strength of the binding of myosin-II filaments to the actin network allows exerting high enough forces to unbind actin/fascin crosslinks. This is shown by varying the binding strength of the acto-myosin bond and by combining the experiments with phenomenological simulations based on simple interaction rules.
自组织机制对于细胞骨架适应活细胞的要求至关重要。它们依赖于细胞骨架丝、交联蛋白和分子马达的复杂相互作用。在这里，我们提出了一个由肌动蛋白丝、细丝蛋白和肌球蛋白 II 丝组成的体外最小模型系统，该系统表现出脉动的集体动力学和超扩散输运性质。这两种现象都依赖于网络中交联分子和马达丝的复杂竞争。只有当肌球蛋白 II 丝与肌动球蛋白网络的结合强度足以产生足以使肌动蛋白/细丝交联解联的高力时，才会观察到这两种现象。这可以通过改变肌球蛋白 II 丝与肌动球蛋白结合的结合强度并结合基于简单相互作用规则的实验和现象模拟来证明。
