Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, PR China.
Oncol Rep. 2011 Dec;26(6):1441-6. doi: 10.3892/or.2011.1442. Epub 2011 Aug 31.
This study was designed to investigate the effect of pseudo-G-Rh2, a novel metabolite of ginsenoside Rh2, on the apoptosis of SGC-7901 human gastric cancer cells. Pseudo-G-Rh2 demonstrated antitumor activity and significantly inhibited the proliferation of SGC-7901 cells in a concentration-dependent manner. After treatment with pseudo-G-Rh2, SGC-7901 cells showed typical apoptotic morphological features, such as chromatin condensation and DNA fragmentation. Pseudo-G-Rh2 could induce mitochondrial membrane potential loss, which led to the release of cytochrome c (Cyt c), Smac/Diablo and apoptosis-inducing factor (AIF) to the cell cytoplasm. Furthermore, pseudo-G-Rh2 exposure not only decreased the expression of the Bcl-2 protein but also increased the expression of the Bax protein and the activities of caspase-9 and caspase-3 in SGC-7901 cells. These results demonstrated that pseudo-G-Rh2 inhibited the proliferation of SGC-7901 cells by initiating apoptosis. Pseudo-G-Rh2-induced apoptosis was associated with a drop in the mitochondrial transmembrane potential, down-regulation of Bcl-2, up-regulation of Bax and activation of caspase-9 and caspase-3.
本研究旨在探讨伪-G-Rh2（人参皂苷 Rh2 的一种新型代谢产物）对人胃癌 SGC-7901 细胞凋亡的影响。伪-G-Rh2 具有抗肿瘤活性，能显著抑制 SGC-7901 细胞的增殖，且呈浓度依赖性。经伪-G-Rh2 处理后，SGC-7901 细胞出现典型的凋亡形态学特征，如染色质浓缩和 DNA 片段化。伪-G-Rh2 可诱导线粒体膜电位丧失，导致细胞色素 c（Cyt c）、Smac/Diablo 和凋亡诱导因子（AIF）释放到细胞质中。此外，伪-G-Rh2 暴露不仅降低了 Bcl-2 蛋白的表达，还增加了 Bax 蛋白的表达以及 SGC-7901 细胞中 caspase-9 和 caspase-3 的活性。这些结果表明，伪-G-Rh2 通过诱导细胞凋亡来抑制 SGC-7901 细胞的增殖。伪-G-Rh2 诱导的细胞凋亡与线粒体跨膜电位下降、Bcl-2 下调、Bax 上调以及 caspase-9 和 caspase-3 的激活有关。
