Institute of Biological Chemistry, Academia Sinica, No. 128 Academia Road Section 2, Nan-Kang, Taipei 11529, Taiwan.
Chembiochem. 2011 Oct 17;12(15):2306-9. doi: 10.1002/cbic.201100417. Epub 2011 Sep 2.
Cellular redox conditions affect Gsp amidase activity in Escherichia coli. Guided by the structure and catalytic mechanism of the amidase, we designed and synthesized an acyloxymethyl ketone-based activity probe containing a biotin handle. This probe was used to monitor Gsp amidase activity in E. coli lysates that had been subjected to oxidative or methylglyoxal-induced stress.
细胞氧化还原条件影响大肠杆菌中的 Gsp 酰胺酶活性。根据酰胺酶的结构和催化机制，我们设计并合成了一种基于酰氧甲基酮的活性探针，其中包含一个生物素接头。该探针用于监测经历氧化或甲基乙二醛诱导应激的大肠杆菌裂解物中的 Gsp 酰胺酶活性。
