Sahin Emel, Goçmen Ayşe Yeşim, Gümüşlü Saadet, Sahin Mehmet, Koçak Hüseyin, Tuncer Murat
Department of Central Laboratory, Clinical Biochemistry Unit, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkey.
Clin Lab. 2011;57(7-8):551-7.
The aim of this study was to examine the alterations in calcium and lipid peroxidation in red blood cells (RBCs) and serum samples of continuous ambulatory peritoneal dialysis (CAPD) patients. We also investigated the relationship between parathyroid hormone (PTH) and calcium homeostasis in this study.
For this purpose, routine blood counts and blood chemistry were analyzed by standard laboratory procedures in serum samples. The concentration of TBARS was measured in erythrocytes and serum samples. RBC calcium was measured by Fura-2AM in a spectrofluorometer.
In CAPD patients, hemoglobin, albumin, and high density lipoprotein cholesterol levels were lower, but glucose, very low density lipoprotein cholesterol, triglyceride, magnesium, PTH, sensitive C-reactive protein, and uric acid levels were higher than the controls. Thiobarbituric acid-reactive substance (TBARS) levels in RBCs and serum samples and cytosolic calcium in RBCs were all found to be significantly increased in CAPD patients compared to control subjects. Multiple regression analysis showed that RBC TBARS and serum PTH were the independent predictors of RBC calcium in our study.
Our results confirm that oxidative stress is an important risk factor for CAPD. The results of multiple regression analysis suggest that RBC calcium was affected by both increased levels of TBARS and PTH.
本研究旨在检测持续性非卧床腹膜透析（CAPD）患者红细胞（RBC）及血清样本中钙和脂质过氧化的变化情况。本研究还探讨了甲状旁腺激素（PTH）与钙稳态之间的关系。
为此，通过标准实验室程序对血清样本进行常规血细胞计数和血液化学分析。测定红细胞和血清样本中硫代巴比妥酸反应物（TBARS）的浓度。用Fura-2AM在荧光分光光度计中测量红细胞钙。
CAPD患者的血红蛋白、白蛋白和高密度脂蛋白胆固醇水平较低，但葡萄糖、极低密度脂蛋白胆固醇、甘油三酯、镁、PTH、超敏C反应蛋白和尿酸水平高于对照组。与对照组相比，CAPD患者红细胞和血清样本中的硫代巴比妥酸反应物质（TBARS）水平以及红细胞中的胞质钙均显著升高。多元回归分析表明，在本研究中红细胞TBARS和血清PTH是红细胞钙的独立预测因子。
我们的结果证实氧化应激是CAPD的一个重要危险因素。多元回归分析结果表明，红细胞钙受TBARS和PTH水平升高的影响。
