Laboratoire Suisse d'Analyse du Dopage, Centre Universitaire Romand de Médecine Légale, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Epalinges, Switzerland.
Forensic Sci Int. 2011 Dec 10;213(1-3):101-3. doi: 10.1016/j.forsciint.2011.07.053. Epub 2011 Sep 1.
C.E.R.A. (Continuous Erythropoietin Receptor Activator) is a new third-generation erythropoiesis-stimulating agent that has recently been linked with abuse in endurance sports. The anti-doping community rapidly reacted by releasing a high-throughput screening ELISA allowing the detection of C.E.R.A. doping in athletes' blood. In order to return adverse analytical findings, anti-doping laboratories, however, need, as far as possible, to confirm the presence of the drug in athletes' samples through orthogonal methods. This article focuses on the comparison of 2 proposed confirmation assays based on gel electrophoresis that were coupled with a new sample immunopurification method. IEF, the classical method used to target erythropoietin (EPO) and its recombinant analogues in athletes' samples, and SARKOSYL-PAGE were applied to the plasma samples of subjects having received a single injection of C.E.R.A. It was demonstrated that SARKOSYL-PAGE was at least 6 times more sensitive than IEF, with comparable specificity. A longer detection window coupled with easier interpretation criteria led us to recommend the use of SARKOSYL-PAGE to confirm C.E.R.A. presence in athletes' blood.
C.E.R.A.（持续红细胞生成素受体激活剂）是一种新的第三代促红细胞生成素刺激剂，最近与耐力运动中的滥用有关。反兴奋剂界迅速做出反应，发布了一种高通量筛选 ELISA，允许检测运动员血液中的 C.E.R.A. 兴奋剂。然而，为了回报不良分析结果，反兴奋剂实验室需要尽可能通过正交方法确认运动员样本中药物的存在。本文重点介绍了两种基于凝胶电泳的拟议确证检测方法的比较，这些方法与一种新的样本免疫纯化方法相结合。IEF 是一种经典的方法，用于靶向运动员样本中的促红细胞生成素 (EPO) 及其重组类似物，而 SARKOSYL-PAGE 则应用于接受单次 C.E.R.A. 注射的受试者的血浆样本。结果表明，SARKOSYL-PAGE 的灵敏度至少比 IEF 高 6 倍，且特异性相当。更长的检测窗口期和更简单的解释标准使我们建议使用 SARKOSYL-PAGE 来确认运动员血液中 C.E.R.A. 的存在。
