Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
Bioorg Med Chem Lett. 2011 Oct 15;21(20):6131-4. doi: 10.1016/j.bmcl.2011.08.026. Epub 2011 Aug 11.
Here we present first dinucleotide affinity resins for purification of proteins that specifically recognize the 5' end of mRNA. Constructed resins possess either a naturally occurring mono- or trimethylated cap or their analogues resistant towards enzymatic degradation, bearing a CH(2) bridge between β and γ position of the 5',5'-triphosphate chain. All cap analogues were attached to a polymer support (EAH-Sepharose) through the carboxylic group that had been generated by derivatization of the 2',3'-cis diol of the second nucleotide in the cap structure with levulinic acid.
这里我们展示了用于纯化特异性识别 mRNA 5' 端的蛋白质的首个二核苷酸亲和树脂。构建的树脂具有天然存在的单或三甲基化帽或对酶降解具有抗性的类似物，在 5'、5'-三磷酸链的β和γ位置之间具有 CH(2)桥。所有帽类似物都通过羧酸基团连接到聚合物载体（EAH-Sepharose）上，羧酸基团是通过用戊酸对帽结构中第二个核苷酸的 2'、3'-顺二醇进行衍生化生成的。
