College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
FEBS Lett. 2011 Oct 3;585(19):3079-85. doi: 10.1016/j.febslet.2011.08.034. Epub 2011 Aug 30.
Small non-coding RNAs regulate gene expression in a sequence-specific manner. In Drosophila, Dicer-2 (Dcr-2) functions in the biogenesis of endogenous small interfering RNAs (endo-siRNAs). We identified 21 distinct proteins that exhibited a ≥ 1.5-fold change as a consequence of loss of dcr-2 function. Most of these were metabolic genes implicated in stress resistance and aging. dcr-2 Mutants had reduced lifespan and were hypersensitive to oxidative, endoplasmic reticulum, starvation, and cold stresses. Furthermore, loss of dcr-2 function led to abnormal lipid and carbohydrate metabolism. Our results suggest roles for the endo-siRNA pathway in metabolic regulation and defense against stress and aging in Drosophila.
小非编码 RNA 以序列特异性方式调节基因表达。在果蝇中，Dicer-2（Dcr-2）在内源性小干扰 RNA（endo-siRNA）的生物发生中发挥作用。我们鉴定了 21 种不同的蛋白质，它们的功能丧失导致了≥1.5 倍的变化。这些蛋白质大多是与应激抵抗和衰老有关的代谢基因。dcr-2 突变体的寿命缩短，对氧化、内质网、饥饿和寒冷应激更为敏感。此外，dcr-2 功能的丧失导致脂质和碳水化合物代谢异常。我们的结果表明，endo-siRNA 途径在果蝇的代谢调控以及应激和衰老防御中发挥作用。
