果蝇内源性 siRNA 通路通过调节代谢稳态来影响应激抗性和寿命。

The endogenous siRNA pathway in Drosophila impacts stress resistance and lifespan by regulating metabolic homeostasis.

机构信息

College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.

出版信息

FEBS Lett. 2011 Oct 3;585(19):3079-85. doi: 10.1016/j.febslet.2011.08.034. Epub 2011 Aug 30.

DOI:10.1016/j.febslet.2011.08.034

Abstract

Small non-coding RNAs regulate gene expression in a sequence-specific manner. In Drosophila, Dicer-2 (Dcr-2) functions in the biogenesis of endogenous small interfering RNAs (endo-siRNAs). We identified 21 distinct proteins that exhibited a ≥ 1.5-fold change as a consequence of loss of dcr-2 function. Most of these were metabolic genes implicated in stress resistance and aging. dcr-2 Mutants had reduced lifespan and were hypersensitive to oxidative, endoplasmic reticulum, starvation, and cold stresses. Furthermore, loss of dcr-2 function led to abnormal lipid and carbohydrate metabolism. Our results suggest roles for the endo-siRNA pathway in metabolic regulation and defense against stress and aging in Drosophila.

摘要

小非编码 RNA 以序列特异性方式调节基因表达。在果蝇中，Dicer-2（Dcr-2）在内源性小干扰 RNA（endo-siRNA）的生物发生中发挥作用。我们鉴定了 21 种不同的蛋白质，它们的功能丧失导致了≥1.5 倍的变化。这些蛋白质大多是与应激抵抗和衰老有关的代谢基因。dcr-2 突变体的寿命缩短，对氧化、内质网、饥饿和寒冷应激更为敏感。此外，dcr-2 功能的丧失导致脂质和碳水化合物代谢异常。我们的结果表明，endo-siRNA 途径在果蝇的代谢调控以及应激和衰老防御中发挥作用。