Kim Chae Jeong, Kim Hyun Ho, Kim Hee Kyung, Lee Sojeong, Jang Daegyu, Kim Chanhyeok, Lim Do-Hwan
School of Systems Biomedical Science, Soongsil University, Seoul 06978, Republic of Korea.
Biology (Basel). 2023 Aug 7;12(8):1096. doi: 10.3390/biology12081096.
Basement membranes (BMs) play important roles under various physiological conditions in animals, including ecdysozoans. During development, BMs undergo alterations through diverse intrinsic and extrinsic regulatory mechanisms; however, the full complement of pathways controlling these changes remain unclear. Here, we found that fat body-overexpression of , which is highly expressed during the larval-to-pupal transition, resulted in a decrease in the overall size of the larval fat body, and ultimately, in a severe growth defect accompanied by a reduction in cell proliferation and cell size. Interestingly, we further observed that a large proportion of the larval fat body cells were prematurely disassociated from each other. Moreover, we present evidence that miR-263b-5p suppresses the main component of BMs, (). Through experiments using RNA interference (RNAi) of , we found that its depletion phenocopied the effects in -overexpressing flies. Overall, our findings suggest a potential role for miR-263b in developmental growth and cell association by suppressing expression in the fat body.
基底膜（BMs）在包括蜕皮动物在内的动物的各种生理条件下发挥着重要作用。在发育过程中，基底膜通过多种内在和外在调节机制发生改变；然而，控制这些变化的完整途径仍不清楚。在这里，我们发现，在幼虫到蛹的转变过程中高度表达的[具体基因名称未给出]在脂肪体中过表达，导致幼虫脂肪体的整体大小减小，最终导致严重的生长缺陷，伴随着细胞增殖和细胞大小的减少。有趣的是，我们进一步观察到很大一部分幼虫脂肪体细胞彼此过早分离。此外，我们提供证据表明miR - 263b - 5p抑制基底膜的主要成分[具体成分名称未给出]。通过使用[具体基因名称未给出]的RNA干扰（RNAi）实验，我们发现其缺失模拟了在[具体基因名称未给出]过表达果蝇中的效应。总体而言，我们的研究结果表明miR - 263b通过抑制脂肪体中[具体基因名称未给出]的表达，在发育生长和细胞关联中发挥潜在作用。
