寡脱氧核苷酸硫代磷酸酯在亚细胞提取物、培养基、血清和脑脊液中的稳定性。
Oligodeoxynucleoside phosphorothioate stability in subcellular extracts, culture media, sera and cerebrospinal fluid.
作者信息
Campbell J M, Bacon T A, Wickstrom E
机构信息
Department of Chemistry, University of South Florida, Tampa 33620.
出版信息
J Biochem Biophys Methods. 1990 Mar;20(3):259-67. doi: 10.1016/0165-022x(90)90084-p.
Degradation of a synthetic oligodeoxynucleoside phosphorothioate was studied in six systems used for antisense inhibition experiments. Oligodeoxynucleoside phosphorothioates were degraded very slowly at 37 degrees C in all of the systems studied. Measured half-lives of pentadecamers were 12 +/- 1 h in rabbit reticulocyte lysate, 7 +/- 1 h in HeLa cell postmitochondrial extract, 14 +/- 2 h in RPMI 1640 with 10% fetal bovine serum, 8 +/- 1 h in undiluted fetal bovine serum, 9 +/- 1 h in adult human serum, and 19 +/- 7 h in rat cerebrospinal fluid.
在用于反义抑制实验的六个系统中研究了合成寡脱氧核苷酸硫代磷酸酯的降解情况。在所研究的所有系统中，寡脱氧核苷酸硫代磷酸酯在37℃时降解非常缓慢。十五聚体在兔网织红细胞裂解物中的测量半衰期为12±1小时，在HeLa细胞线粒体后提取物中为7±1小时，在含10%胎牛血清的RPMI 1640中为14±2小时，在未稀释的胎牛血清中为8±1小时，在成人血清中为9±1小时，在大鼠脑脊液中为19±7小时。
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