Toba M, Suzuki H, Sekine N
Intervirology. 1979;11(4):221-6. doi: 10.1159/000149037.
A cell line established from human embryonic lung, HEL-R66, was demonstrated to be highly susceptible to herpes simplex virus types 1 and 2, vaccinia virus, Newcastle disease virus (NDV), Japanese encephalitis virus (JEV), western equine encephalitis (WEE) virus, Sindbis virus, vesicular stomatitis virus (VSV), and rabies virus. The maximal yields of NDV, JEV, WEE virus, and rabies virus in this cell line exceeded by 2--4 logs those in control human embryonic lung cells. Inability of this cell line to produce interferon upon treatment with native and UV-irradiated forms of virogenic and lentogenic strains of NDV and with poly I:C was revealed. A refractory state to challenging VSV did not develop in HEL-R66 cells treated with the inducers. Furthermore, pretreatment of HEL-R66 cells with interferon did not potentiate the capacity to produce interferon in response to the addition of poly I:C, whereas the same treatment enhanced the production of interferon in normal human embryonic lung cells.
一种源自人胚肺的细胞系HEL-R66，已被证明对1型和2型单纯疱疹病毒、痘苗病毒、新城疫病毒（NDV）、日本脑炎病毒（JEV）、西部马脑炎（WEE）病毒、辛德毕斯病毒、水疱性口炎病毒（VSV）和狂犬病病毒高度敏感。该细胞系中NDV、JEV、WEE病毒和狂犬病病毒的最大产量比对照人胚肺细胞中的产量高出2至4个对数级。研究发现，用天然和紫外线照射形式的NDV病毒ogenic和lentogenic株以及聚肌胞苷酸（poly I:C）处理该细胞系时，它无法产生干扰素。在用诱导剂处理的HEL-R66细胞中，未出现对VSV攻击的不应答状态。此外，用干扰素预处理HEL-R66细胞并不能增强其在添加聚肌胞苷酸后产生干扰素的能力，而相同处理却能增强正常人胚肺细胞中干扰素的产生。
